Oligodendrocytes regulate formation of nodes of Ranvier via the recognition molecule OMgp

Du Yu Nie; Quan Hong Ma; Janice W S Law; Chern Pang Chia; Narender K. Dhingra; Yasushi Shimoda; Wu Lin Yang; Neng Gong; Qing Wen Chen; Gang Xu; Qi Dong Hu; Pierce K H Chow; Yee Kong Ng; Eng Ang Ling; Kazutada Watanabe; Tian Le Xu; Amyn A. Habib; Melitta Schachner; Zhi Cheng Xiao

doi:10.1017/S1740925X06000251

Oligodendrocytes regulate formation of nodes of Ranvier via the recognition molecule OMgp

Du Yu Nie, Quan Hong Ma, Janice W S Law, Chern Pang Chia, Narender K. Dhingra, Yasushi Shimoda, Wu Lin Yang, Neng Gong, Qing Wen Chen, Gang Xu, Qi Dong Hu, Pierce K H Chow, Yee Kong Ng, Eng Ang Ling, Kazutada Watanabe, Tian Le Xu, Amyn A. Habib, Melitta Schachner, Zhi Cheng Xiao

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The molecular mechanisms underlying the involvement of oligodendrocytes in formation of the nodes of Ranvier (NORs) remain poorly understood. Here we show that oligodendrocyte-myelin glycoprotein (OMgp) aggregates specifically at NORs. Nodal location of OMgp does not occur along demyelinated axons of either Shiverer or proteolipid protein (PLP) transgenic mice. Over-expression of OMgp in OLN-93 cells facilitates process outgrowth. In transgenic mice in which expression of OMgp is down-regulated, myelin thickness declines, and lateral oligodendrocyte loops at the node-paranode junction are less compacted and even join together with the opposite loops, which leads to shortened nodal gaps. Notably, each of these structural abnormalities plus modest down-regulation of expression of Na⁺ channel α subunit result in reduced conduction velocity in the spinal cords of the mutant mice. Thus, OMgp that is derived from glia has distinct roles in regulating nodal formation and function during CNS myelination.

Original language	English (US)
Pages (from-to)	151-164
Number of pages	14
Journal	Neuron Glia Biology
Volume	2
Issue number	3
DOIs	https://doi.org/10.1017/S1740925X06000251
State	Published - Aug 2006

Keywords

Myelination
Na channel
Node of Ranvier
OMgp
Oligodendrocyte

ASJC Scopus subject areas

Cellular and Molecular Neuroscience
Cell Biology

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10.1017/S1740925X06000251

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Nie, D. Y., Ma, Q. H., Law, J. W. S., Chia, C. P., Dhingra, N. K., Shimoda, Y., Yang, W. L., Gong, N., Chen, Q. W., Xu, G., Hu, Q. D., Chow, P. K. H., Ng, Y. K., Ling, E. A., Watanabe, K., Xu, T. L., Habib, A. A., Schachner, M., & Xiao, Z. C. (2006). Oligodendrocytes regulate formation of nodes of Ranvier via the recognition molecule OMgp. Neuron Glia Biology, 2(3), 151-164. https://doi.org/10.1017/S1740925X06000251

Nie, DY, Ma, QH, Law, JWS, Chia, CP, Dhingra, NK, Shimoda, Y, Yang, WL, Gong, N, Chen, QW, Xu, G, Hu, QD, Chow, PKH, Ng, YK, Ling, EA, Watanabe, K, Xu, TL, Habib, AA, Schachner, M & Xiao, ZC 2006, 'Oligodendrocytes regulate formation of nodes of Ranvier via the recognition molecule OMgp', Neuron Glia Biology, vol. 2, no. 3, pp. 151-164. https://doi.org/10.1017/S1740925X06000251

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title = "Oligodendrocytes regulate formation of nodes of Ranvier via the recognition molecule OMgp",

abstract = "The molecular mechanisms underlying the involvement of oligodendrocytes in formation of the nodes of Ranvier (NORs) remain poorly understood. Here we show that oligodendrocyte-myelin glycoprotein (OMgp) aggregates specifically at NORs. Nodal location of OMgp does not occur along demyelinated axons of either Shiverer or proteolipid protein (PLP) transgenic mice. Over-expression of OMgp in OLN-93 cells facilitates process outgrowth. In transgenic mice in which expression of OMgp is down-regulated, myelin thickness declines, and lateral oligodendrocyte loops at the node-paranode junction are less compacted and even join together with the opposite loops, which leads to shortened nodal gaps. Notably, each of these structural abnormalities plus modest down-regulation of expression of Na+ channel α subunit result in reduced conduction velocity in the spinal cords of the mutant mice. Thus, OMgp that is derived from glia has distinct roles in regulating nodal formation and function during CNS myelination.",

keywords = "Myelination, Na channel, Node of Ranvier, OMgp, Oligodendrocyte",

author = "Nie, {Du Yu} and Ma, {Quan Hong} and Law, {Janice W S} and Chia, {Chern Pang} and Dhingra, {Narender K.} and Yasushi Shimoda and Yang, {Wu Lin} and Neng Gong and Chen, {Qing Wen} and Gang Xu and Hu, {Qi Dong} and Chow, {Pierce K H} and Ng, {Yee Kong} and Ling, {Eng Ang} and Kazutada Watanabe and Xu, {Tian Le} and Habib, {Amyn A.} and Melitta Schachner and Xiao, {Zhi Cheng}",

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doi = "10.1017/S1740925X06000251",

language = "English (US)",

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AU - Nie, Du Yu

AU - Ma, Quan Hong

AU - Law, Janice W S

AU - Chia, Chern Pang

AU - Dhingra, Narender K.

AU - Shimoda, Yasushi

AU - Yang, Wu Lin

AU - Gong, Neng

AU - Chen, Qing Wen

AU - Xu, Gang

AU - Hu, Qi Dong

AU - Chow, Pierce K H

AU - Ng, Yee Kong

AU - Ling, Eng Ang

AU - Watanabe, Kazutada

AU - Xu, Tian Le

AU - Habib, Amyn A.

AU - Schachner, Melitta

AU - Xiao, Zhi Cheng

PY - 2006/8

Y1 - 2006/8

N2 - The molecular mechanisms underlying the involvement of oligodendrocytes in formation of the nodes of Ranvier (NORs) remain poorly understood. Here we show that oligodendrocyte-myelin glycoprotein (OMgp) aggregates specifically at NORs. Nodal location of OMgp does not occur along demyelinated axons of either Shiverer or proteolipid protein (PLP) transgenic mice. Over-expression of OMgp in OLN-93 cells facilitates process outgrowth. In transgenic mice in which expression of OMgp is down-regulated, myelin thickness declines, and lateral oligodendrocyte loops at the node-paranode junction are less compacted and even join together with the opposite loops, which leads to shortened nodal gaps. Notably, each of these structural abnormalities plus modest down-regulation of expression of Na+ channel α subunit result in reduced conduction velocity in the spinal cords of the mutant mice. Thus, OMgp that is derived from glia has distinct roles in regulating nodal formation and function during CNS myelination.

AB - The molecular mechanisms underlying the involvement of oligodendrocytes in formation of the nodes of Ranvier (NORs) remain poorly understood. Here we show that oligodendrocyte-myelin glycoprotein (OMgp) aggregates specifically at NORs. Nodal location of OMgp does not occur along demyelinated axons of either Shiverer or proteolipid protein (PLP) transgenic mice. Over-expression of OMgp in OLN-93 cells facilitates process outgrowth. In transgenic mice in which expression of OMgp is down-regulated, myelin thickness declines, and lateral oligodendrocyte loops at the node-paranode junction are less compacted and even join together with the opposite loops, which leads to shortened nodal gaps. Notably, each of these structural abnormalities plus modest down-regulation of expression of Na+ channel α subunit result in reduced conduction velocity in the spinal cords of the mutant mice. Thus, OMgp that is derived from glia has distinct roles in regulating nodal formation and function during CNS myelination.

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Oligodendrocytes regulate formation of nodes of Ranvier via the recognition molecule OMgp

Abstract

Keywords

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