On-bead combinatorial synthesis and imaging of europium(III)-based paraCEST agents aids in identification of chemical features that enhance CEST sensitivity

Jaspal Singh, Vineeta Rustagi, Shanrong Zhang, A. Dean Sherry, D. Gomika Udugamasooriya

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The rate of water exchange between the inner sphere of a paramagnetic ion and bulk water is an important parameter in determining the magnitude of the chemical exchange saturation transfer signal from paramagnetic CEST agents (paraCEST). This is governed by various geometric, steric and ligand field factors created by macrocyclic ligands surrounding the paramagnetic metal ion. Our previous on-bead combinatorial studies of di-peptoid-europium(III)-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA)-tetraamide complexes revealed that negatively charged groups in the immediate vicinity of the metal center strongly enhances the CEST signal. Here, we report a solid phase synthesis and on-bead imaging of 76 new DOTA derivatives that are developed by coupling with a single residue onto each of the three arms of a DOTA-tetraamide scaffold attached to resin beads. This single residue predominantly carries negatively charged groups blended with various physico-chemical characteristics. We found that non-bulky negatively charged groups are best suited at the immediate vicinity of the metal ion, while positive, bulky and halogen containing moieties suppress the CEST signal.

Original languageEnglish (US)
JournalMagnetic Resonance in Chemistry
DOIs
StateAccepted/In press - 2017

Fingerprint

Europium
Metal ions
Peptoids
Ligands
Imaging techniques
Halogens
Water
Scaffolds
Ion exchange
Resins
Metals
Ions
Derivatives
Acids
1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid

Keywords

  • CEST-MRI
  • Combinatorial
  • DOTA

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)

Cite this

On-bead combinatorial synthesis and imaging of europium(III)-based paraCEST agents aids in identification of chemical features that enhance CEST sensitivity. / Singh, Jaspal; Rustagi, Vineeta; Zhang, Shanrong; Sherry, A. Dean; Udugamasooriya, D. Gomika.

In: Magnetic Resonance in Chemistry, 2017.

Research output: Contribution to journalArticle

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