We report a novel approach that generates a high resolution, three-dimensional (3D) fluorescent staining atlas of tissue microbiopsies in a microfluidic device without destroying the tissue. We demonstrate that this method preserves tissue architecture for multiple murine organs by comparing traditional 2D slices to an optically sectioned 3D H&E-mimic. The H&E-mimic slices show a close qualitative match to traditional H&E. The 3D spatial and molecular information obtainable from this method significantly increases the amount of data available for evaluating both tissue morphology and specific biomarkers in a wide range of both research and clinically driven applications and is amenable to automation.
ASJC Scopus subject areas
- Biomedical Engineering
- Materials Science(all)
- Condensed Matter Physics
- Fluid Flow and Transfer Processes
- Colloid and Surface Chemistry