On-chip three-dimensional tissue histology for microbiopsies

Cody Narciso, Kyle R. Cowdrick, Victoria Zellmer, Teresa Brito-Robinson, Pavel Brodskiy, David J. Hoelzle, Siyuan Zhang, Jeremiah J. Zartman

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

We report a novel approach that generates a high resolution, three-dimensional (3D) fluorescent staining atlas of tissue microbiopsies in a microfluidic device without destroying the tissue. We demonstrate that this method preserves tissue architecture for multiple murine organs by comparing traditional 2D slices to an optically sectioned 3D H&E-mimic. The H&E-mimic slices show a close qualitative match to traditional H&E. The 3D spatial and molecular information obtainable from this method significantly increases the amount of data available for evaluating both tissue morphology and specific biomarkers in a wide range of both research and clinically driven applications and is amenable to automation.

Original languageEnglish (US)
Article number021101
JournalBiomicrofluidics
Volume10
Issue number2
DOIs
StatePublished - Mar 1 2016
Externally publishedYes

ASJC Scopus subject areas

  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics
  • Fluid Flow and Transfer Processes
  • Colloid and Surface Chemistry

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