TY - JOUR
T1 - On the pivotal role of Elovl3/ELOVL3 in meibogenesis and ocular physiology of mice
AU - Butovich, Igor A.
AU - Wilkerson, Amber
AU - Bhat, Nita
AU - McMahon, Anne
AU - Yuksel, Seher
N1 - Funding Information:
This work was supported, in part, by U.S. National Institutes of Health, National Eye Institute Grant R01EY024324 (to I.A.B.), an unrestricted grant from Research to Prevent Blindness (New York, NY, USA), and also, in part, by a 2219‐International Postdoctoral Research Scholarship from the Scientific and Technological Research Council of Turkey (TÜBITAK; Ankara, Turkey) to S.Y. The authors declare no conflicts of interest.
Publisher Copyright:
© FASEB
PY - 2019/9/1
Y1 - 2019/9/1
N2 - The purpose of this study was to examine the role of Elovl3 gene in meibogenesis and the impact of ELOVL3 protein ablation on the physiology of the mouse ocular surface and Meibomian glands (MGs). Elovl3 knockout, ELOVL3-ablated (E3hom) mice and their wild type littermates (E3wt) were studied side by side. E3hom mice had abnormal ocular phenotypes such as delayed eye opening, weeping eyes, crusty eyelids, eyelid edema, highly vascularized cornea and tarsal plates (TPs), slit eye, and increased tearing that resemble symptoms observed in human subjects with various forms of dry eye, MG dysfunction and blepharitis. Lipid profiling of E3hom TPs was conducted using chromatography and mass spectrometry. The analyses revealed that the lipid composition of E3hom TPs was strikingly different from that of their E3wt littermates. The mutation affected major classes of meibomian lipids - cholesteryl esters, wax esters, and cholesteryl esters of (O)-acylated w-hydroxy fatty acids. The studies illuminated the central role of ELOVL3 in producing C21:0-C29:0 fatty acids, including odd-chain and branched ones. Ablation of ELOVL3 leads to selective changes in the lipid composition of meibum, making E3hom mice instrumental in studying the mechanisms of the biosynthesis of meibum and modeling various pathologies of human ocular surface and adnexa.—Butovich, I. A., Wilkerson, A., Bhat, N., McMahon, A., Yuksel, S. On the pivotal role of Elovl3/ELOVL3 in meibogenesis and ocular physiology of mice. FASEB J. 33, 10034–10048 (2019). www.fasebj.org.
AB - The purpose of this study was to examine the role of Elovl3 gene in meibogenesis and the impact of ELOVL3 protein ablation on the physiology of the mouse ocular surface and Meibomian glands (MGs). Elovl3 knockout, ELOVL3-ablated (E3hom) mice and their wild type littermates (E3wt) were studied side by side. E3hom mice had abnormal ocular phenotypes such as delayed eye opening, weeping eyes, crusty eyelids, eyelid edema, highly vascularized cornea and tarsal plates (TPs), slit eye, and increased tearing that resemble symptoms observed in human subjects with various forms of dry eye, MG dysfunction and blepharitis. Lipid profiling of E3hom TPs was conducted using chromatography and mass spectrometry. The analyses revealed that the lipid composition of E3hom TPs was strikingly different from that of their E3wt littermates. The mutation affected major classes of meibomian lipids - cholesteryl esters, wax esters, and cholesteryl esters of (O)-acylated w-hydroxy fatty acids. The studies illuminated the central role of ELOVL3 in producing C21:0-C29:0 fatty acids, including odd-chain and branched ones. Ablation of ELOVL3 leads to selective changes in the lipid composition of meibum, making E3hom mice instrumental in studying the mechanisms of the biosynthesis of meibum and modeling various pathologies of human ocular surface and adnexa.—Butovich, I. A., Wilkerson, A., Bhat, N., McMahon, A., Yuksel, S. On the pivotal role of Elovl3/ELOVL3 in meibogenesis and ocular physiology of mice. FASEB J. 33, 10034–10048 (2019). www.fasebj.org.
KW - fatty acid elongation
KW - meibomian glands
KW - meibum
KW - tarsal plates
UR - http://www.scopus.com/inward/record.url?scp=85071783887&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85071783887&partnerID=8YFLogxK
U2 - 10.1096/fj.201900725R
DO - 10.1096/fj.201900725R
M3 - Article
C2 - 31208226
AN - SCOPUS:85071783887
SN - 0892-6638
VL - 33
SP - 10034
EP - 10048
JO - FASEB Journal
JF - FASEB Journal
IS - 9
ER -