On the role of Eph signalling in thymus histogenesis; EphB2/B3 and the organizing of the thymic epithelial network

Javier García-Ceca, Eva Jiménez, David Alfaro, Teresa Cejalvo, Michael J. Chumley, Mark Henkemeyer, Juan José Muñoz, Agustín G. Zapata

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In the current study, we extend our own previous results on the thymocyte phenotype of EphB2 and/or EphB3 deficient mice by analyzing the phenotype and the histological organization of their thymic epithelial stroma. All studied adult EphB-deficient thymi showed profound alterations with respect to the wild-type (WT) ones. Each mutant exhibited a specific phenotype, but also showed common features including occurrence of K5+K8+MTS10 + immature medullary epithelial cells, numerous K5-K8 -MTS20+ cells and K5+K8+ cells in the thymic cortex and cortical and medullary K5-K8- areas devoid of epithelial cell markers. In addition, comparative analysis of WT and EphB-deficient embryonic and newborn thymi demonstrated that the observed adult phenotype was a consequence of the gradual accumulation of early phenotypic and morphological defects, becoming more severe at the end of embryonic life and in newborn animals. Together, these results confirm a role for EphB2 and EphB3 in thymus morphogenesis. The obtained data are discussed from the point of view of the recognized role played by these two Ephs in the homeostasis of other epithelia and their possible relationships with molecules known to be involved in thymic epithelial cell development.

Original languageEnglish (US)
Pages (from-to)971-982
Number of pages12
JournalInternational Journal of Developmental Biology
Volume53
Issue number7
DOIs
StatePublished - 2009

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Thymus Gland
Phenotype
Epithelial Cells
Newborn Animals
Thymocytes
Morphogenesis
Homeostasis
Epithelium

Keywords

  • Development
  • Keratin
  • Lymphoid organ
  • Thymic epithelial cell
  • Tyrosine kinase receptor

ASJC Scopus subject areas

  • Developmental Biology
  • Embryology
  • Medicine(all)

Cite this

On the role of Eph signalling in thymus histogenesis; EphB2/B3 and the organizing of the thymic epithelial network. / García-Ceca, Javier; Jiménez, Eva; Alfaro, David; Cejalvo, Teresa; Chumley, Michael J.; Henkemeyer, Mark; Muñoz, Juan José; Zapata, Agustín G.

In: International Journal of Developmental Biology, Vol. 53, No. 7, 2009, p. 971-982.

Research output: Contribution to journalArticle

García-Ceca, Javier ; Jiménez, Eva ; Alfaro, David ; Cejalvo, Teresa ; Chumley, Michael J. ; Henkemeyer, Mark ; Muñoz, Juan José ; Zapata, Agustín G. / On the role of Eph signalling in thymus histogenesis; EphB2/B3 and the organizing of the thymic epithelial network. In: International Journal of Developmental Biology. 2009 ; Vol. 53, No. 7. pp. 971-982.
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