Oncogenic Ras-transformed human fibroblasts exhibit differential changes in contraction and migration in 3D collagen matrices

Gustavo C. Menezes, Miguel Miron-Mendoza, Chin Han Ho, Hongmei Jiang, Frederick Grinnell

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Tractional force exerted by tissue cells in 3D collagen matrices can be utilized for matrix remodeling or cell migration. The interrelationship between these motile processes is not well understood. The current studies were carried out to test the consequences of oncogenic Ras (H-RasV12) transformation on human fibroblast contraction and migration in 3D collagen matrices. Beginning with hTERT-immortalized cells, we prepared fibroblasts stably transformed with E6/E7 and with the combination HPV16 E6/E7 and H-RasV12. Oncogenic Ras-transformed cells lost contact inhibition of cell growth, formed colonies in soft agar and were unable to make adherens junctions. We observed no changes in the extent or growth factor dependence of collagen matrix contraction (floating or stress-relaxation) by oncogenic Ras-transformed cells. On the other hand, transformed cells in nested collagen matrices lost not only growth factor selectivity, but also cell-matrix density-dependent inhibition of migration. These findings demonstrate differential regulation of collagen matrix contraction and cell migration in 3D collagen matrices.

Original languageEnglish (US)
Pages (from-to)3081-3091
Number of pages11
JournalExperimental Cell Research
Volume314
Issue number16
DOIs
StatePublished - Oct 1 2008

Fingerprint

Collagen
Fibroblasts
Cell Movement
Intercellular Signaling Peptides and Proteins
Contact Inhibition
Adherens Junctions
Agar
Cell Count
Growth

Keywords

  • Adherens junctions
  • Collagen
  • Contraction
  • Fibroblast
  • Mechanoregulation
  • Migration
  • Oncogenic Ras

ASJC Scopus subject areas

  • Cell Biology

Cite this

Oncogenic Ras-transformed human fibroblasts exhibit differential changes in contraction and migration in 3D collagen matrices. / Menezes, Gustavo C.; Miron-Mendoza, Miguel; Ho, Chin Han; Jiang, Hongmei; Grinnell, Frederick.

In: Experimental Cell Research, Vol. 314, No. 16, 01.10.2008, p. 3081-3091.

Research output: Contribution to journalArticle

Menezes, Gustavo C. ; Miron-Mendoza, Miguel ; Ho, Chin Han ; Jiang, Hongmei ; Grinnell, Frederick. / Oncogenic Ras-transformed human fibroblasts exhibit differential changes in contraction and migration in 3D collagen matrices. In: Experimental Cell Research. 2008 ; Vol. 314, No. 16. pp. 3081-3091.
@article{4f229364a15d475ba4b5111090f9f5b7,
title = "Oncogenic Ras-transformed human fibroblasts exhibit differential changes in contraction and migration in 3D collagen matrices",
abstract = "Tractional force exerted by tissue cells in 3D collagen matrices can be utilized for matrix remodeling or cell migration. The interrelationship between these motile processes is not well understood. The current studies were carried out to test the consequences of oncogenic Ras (H-RasV12) transformation on human fibroblast contraction and migration in 3D collagen matrices. Beginning with hTERT-immortalized cells, we prepared fibroblasts stably transformed with E6/E7 and with the combination HPV16 E6/E7 and H-RasV12. Oncogenic Ras-transformed cells lost contact inhibition of cell growth, formed colonies in soft agar and were unable to make adherens junctions. We observed no changes in the extent or growth factor dependence of collagen matrix contraction (floating or stress-relaxation) by oncogenic Ras-transformed cells. On the other hand, transformed cells in nested collagen matrices lost not only growth factor selectivity, but also cell-matrix density-dependent inhibition of migration. These findings demonstrate differential regulation of collagen matrix contraction and cell migration in 3D collagen matrices.",
keywords = "Adherens junctions, Collagen, Contraction, Fibroblast, Mechanoregulation, Migration, Oncogenic Ras",
author = "Menezes, {Gustavo C.} and Miguel Miron-Mendoza and Ho, {Chin Han} and Hongmei Jiang and Frederick Grinnell",
year = "2008",
month = "10",
day = "1",
doi = "10.1016/j.yexcr.2008.07.018",
language = "English (US)",
volume = "314",
pages = "3081--3091",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "16",

}

TY - JOUR

T1 - Oncogenic Ras-transformed human fibroblasts exhibit differential changes in contraction and migration in 3D collagen matrices

AU - Menezes, Gustavo C.

AU - Miron-Mendoza, Miguel

AU - Ho, Chin Han

AU - Jiang, Hongmei

AU - Grinnell, Frederick

PY - 2008/10/1

Y1 - 2008/10/1

N2 - Tractional force exerted by tissue cells in 3D collagen matrices can be utilized for matrix remodeling or cell migration. The interrelationship between these motile processes is not well understood. The current studies were carried out to test the consequences of oncogenic Ras (H-RasV12) transformation on human fibroblast contraction and migration in 3D collagen matrices. Beginning with hTERT-immortalized cells, we prepared fibroblasts stably transformed with E6/E7 and with the combination HPV16 E6/E7 and H-RasV12. Oncogenic Ras-transformed cells lost contact inhibition of cell growth, formed colonies in soft agar and were unable to make adherens junctions. We observed no changes in the extent or growth factor dependence of collagen matrix contraction (floating or stress-relaxation) by oncogenic Ras-transformed cells. On the other hand, transformed cells in nested collagen matrices lost not only growth factor selectivity, but also cell-matrix density-dependent inhibition of migration. These findings demonstrate differential regulation of collagen matrix contraction and cell migration in 3D collagen matrices.

AB - Tractional force exerted by tissue cells in 3D collagen matrices can be utilized for matrix remodeling or cell migration. The interrelationship between these motile processes is not well understood. The current studies were carried out to test the consequences of oncogenic Ras (H-RasV12) transformation on human fibroblast contraction and migration in 3D collagen matrices. Beginning with hTERT-immortalized cells, we prepared fibroblasts stably transformed with E6/E7 and with the combination HPV16 E6/E7 and H-RasV12. Oncogenic Ras-transformed cells lost contact inhibition of cell growth, formed colonies in soft agar and were unable to make adherens junctions. We observed no changes in the extent or growth factor dependence of collagen matrix contraction (floating or stress-relaxation) by oncogenic Ras-transformed cells. On the other hand, transformed cells in nested collagen matrices lost not only growth factor selectivity, but also cell-matrix density-dependent inhibition of migration. These findings demonstrate differential regulation of collagen matrix contraction and cell migration in 3D collagen matrices.

KW - Adherens junctions

KW - Collagen

KW - Contraction

KW - Fibroblast

KW - Mechanoregulation

KW - Migration

KW - Oncogenic Ras

UR - http://www.scopus.com/inward/record.url?scp=51649112320&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=51649112320&partnerID=8YFLogxK

U2 - 10.1016/j.yexcr.2008.07.018

DO - 10.1016/j.yexcr.2008.07.018

M3 - Article

C2 - 18708049

AN - SCOPUS:51649112320

VL - 314

SP - 3081

EP - 3091

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 16

ER -