One-step immunoaffinity purification of recombinant human retinoic acid receptor γ

Joyce J. Repa, Jessica A. Berg, Mary E. Kaiser, Kristine K. Hanson, Stephen A. Strugnell, Margaret Clagett-Dame

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Retinoic acid receptors (RAR) are members of the steroid/thyroid hormone receptor superfamily and serve as ligand-activated transcription factors. In order to facilitate studies of receptor protein, we have generated a monoclonal antibody to the human RARγ, and have developed a procedure to purify the full length receptor expressed in insect cells. The monoclonal antibody (A10) was developed using as antigen a carboxy-terminal fragment of the human RARγ expressed as a bacterial fusion protein. The A10 monoclonal antibody binds to both native and denatured forms of the human RARγ. This antibody was immobilized on a resin and used to purify full-length, baculovirus-expressed human RARγ to near homogeneity. The immunoaffinity-purified receptor is >90-95% pure as revealed by silver-stained gels. The identity of the single protein band as RARγ, was verified by immunoblotting using a polyclonal antibody to an epitope distinct from that recognized by the A10 antibody. The pure human RARγ is functional with respect to both ligand and DNA binding. Scatchard analysis of 3H-labeled all-trans retinoic acid binding to purified human RARγ revealed a single, high-affinity binding site with a K(d) of ~2 nM. Binding of the pure RARγ to a DR5-type retinoic acid response element was also studied. Response element binding by RARγ required the presence of the retinoid X receptor, but did not require the presence of additional proteins. Human RARγ protein purified in this fashion will be useful in future structural and functional studies.

Original languageEnglish (US)
Pages (from-to)319-330
Number of pages12
JournalProtein Expression and Purification
Volume9
Issue number3
DOIs
StatePublished - Apr 1997

Fingerprint

Retinoic Acid Receptors
Purification
antineoplaston A10
Monoclonal Antibodies
Response Elements
Tretinoin
Proteins
Antibodies
Immobilized Antibodies
Ligands
Retinoid X Receptors
Thyroid Hormone Receptors
Bacterial Proteins
Baculoviridae
Silver
Immunoblotting
Insects
Epitopes
Transcription Factors
Fusion reactions

ASJC Scopus subject areas

  • Biochemistry

Cite this

One-step immunoaffinity purification of recombinant human retinoic acid receptor γ. / Repa, Joyce J.; Berg, Jessica A.; Kaiser, Mary E.; Hanson, Kristine K.; Strugnell, Stephen A.; Clagett-Dame, Margaret.

In: Protein Expression and Purification, Vol. 9, No. 3, 04.1997, p. 319-330.

Research output: Contribution to journalArticle

Repa, Joyce J. ; Berg, Jessica A. ; Kaiser, Mary E. ; Hanson, Kristine K. ; Strugnell, Stephen A. ; Clagett-Dame, Margaret. / One-step immunoaffinity purification of recombinant human retinoic acid receptor γ. In: Protein Expression and Purification. 1997 ; Vol. 9, No. 3. pp. 319-330.
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