Ontogeny and pathway of formation of 5α-androstane-3α,17β-diol in the testes of the immature brushtail possum Trichosurus vulpecula

Jean D. Wilson, Geoffrey Shaw, Marilyn B. Renfree, Richard J. Auchus, Michael W. Leihy, Douglas C. Eckery

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The testicular androgen 5α-androstane-3α,17β-diol (androstanediol) mediates virilisation in pouch young of a marsupial, the tammar wallaby, and is the principal androgen formed in immature rodent testes. To chart the pattern of androstanediol formation in another marsupial species, the testes or fragments of testes from brushtail possums (Trichosurus vulpecula) that spanned the age range from early pouch young to mature adults were incubated with 3H-progesterone and the products were identified by high-performance liquid chromatography. The only 19-carbon steroids identified in pouch young and adult testes were the Δ 4-3-keto-steroids testosterone and androstenedione. However, androstanediol and another 5α-reduced androgen (androsterone) were synthesised by testes from Day 87-200 males and these appeared to be formed from the 5α-reduction and 3-keto reduction of testosterone and androstenedione. In the prostate and glans penis of the immature male, 3H-androstanediol was converted to dihydrotestosterone. We conclude that the timing of androstanediol formation in the possum testis resembles the process in rodents rather than in the tammar wallaby and that any androstanediol in the circulation probably acts in target tissues via conversion to dihydrotestosterone.

Original languageEnglish (US)
Pages (from-to)603-609
Number of pages7
JournalReproduction, Fertility and Development
Volume17
Issue number6
DOIs
StatePublished - 2005

Fingerprint

Androstane-3,17-diol
Trichosurus
Trichosurus vulpecula
glycols
ontogeny
Testis
testes
immatures
pouches
androgens
Androgens
Macropus eugenii
Macropodidae
Marsupialia
androstenedione
Androstenedione
Dihydrotestosterone
Metatheria
testosterone
steroids

Keywords

  • Dihydrotestosterone
  • Epididymis
  • Marsupial physiology
  • Phallus
  • Prostate
  • Steroid 5α-reductase
  • Virilisation

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Reproductive Medicine
  • Developmental Biology
  • Endocrinology

Cite this

Ontogeny and pathway of formation of 5α-androstane-3α,17β-diol in the testes of the immature brushtail possum Trichosurus vulpecula. / Wilson, Jean D.; Shaw, Geoffrey; Renfree, Marilyn B.; Auchus, Richard J.; Leihy, Michael W.; Eckery, Douglas C.

In: Reproduction, Fertility and Development, Vol. 17, No. 6, 2005, p. 603-609.

Research output: Contribution to journalArticle

Wilson, Jean D. ; Shaw, Geoffrey ; Renfree, Marilyn B. ; Auchus, Richard J. ; Leihy, Michael W. ; Eckery, Douglas C. / Ontogeny and pathway of formation of 5α-androstane-3α,17β-diol in the testes of the immature brushtail possum Trichosurus vulpecula. In: Reproduction, Fertility and Development. 2005 ; Vol. 17, No. 6. pp. 603-609.
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