Abstract
OBJECTIVE: Our purpose was to determine if differences in angiotensin II vascular smooth muscle receptor binding account for the attenuated fetal placental and systemic responses to infused angiotensin II relative to maternal responses and for the differences between umbilical and fetal systemic responses. STUDY DESIGN: Using plasma membranes prepared from the medial layer of fetal aorta, fetal placental artery, and maternal uterine artery obtained between 107 and 134 days of ovine gestation (n = 17), we measured and compared angiotensin II receptor binding density (in femtomoles per milligram of protein) and affinity (in nanomoles per liter) in radioligand binding studies with iodine 125-angiotensin II. Maternal and fetal plasma angiotensin II were also compared. RESULTS: A single class of high-affinity receptor was identified in all arteries. Although the binding density was similar at < 100 and > 130 days for fetal aorta (388 ± 87 [SE] vs 262 ± 56), placental artery (319 ± 95 vs 235 ± 54), and uterine artery (46 ± 6 vs 50 ± 13), values for fetal arteries exceeded those for uterine arteries (p ≤ 0.018) in spite of higher fetal plasma angiotensin II (74 ± 18 vs 30 ± 8 pg/ml, p < 0.01). Affinity did not differ between arteries and, except for fetal aorta (1.4 ± 0.2 vs 2.1 ± 0.4, r = 0.49, p = 0.045), was unchanged during late gestation. CONCLUSION: Differences between fetal and maternal responses and fetal placental and systemic responses to infused angiotensin II in sheep do not reflect alterations in total angiotensin II vascular smooth muscle receptor binding density or affinity.
Original language | English (US) |
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Pages (from-to) | 1562-1569 |
Number of pages | 8 |
Journal | American journal of obstetrics and gynecology |
Volume | 168 |
Issue number | 5 |
DOIs | |
State | Published - 1993 |
Keywords
- Receptors
- angiotensin II
- fetus
- pregnancy
ASJC Scopus subject areas
- Obstetrics and Gynecology