Ontogeny of angiotensin II vascular smooth muscle receptors in ovine fetal aorta and placental and uterine arteries

C. R. Rosenfeld, B. E. Cox, R. R. Magness, P. W. Shaul

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Objective: Our purpose was to determine if differences in angiotensin II vascular smooth muscle receptor binding account for the attenuated fetal placental and systemic responses to infused angiotensin II relative to maternal responses and for the differences between umbilical and fetal systemic responses. Study design: Using plasma membranes prepared from the medial layer of fetal aorta, fetal placental artery, and maternal uterine artery obtained between 107 and 134 days of ovine gestation (n = 17), we measured and compared angiotensin II receptor binding density (in femtomoles per milligram of protein) and affinity (in nanomoles per liter) in radioligand binding studies with iodine 125-angiotensin II. Maternal and fetal plasma angiotensin II were also compared. Results: A single class of high-affinity receptor was identified in all arteries. Although the binding density was similar at < 100 and > 130 days for fetal aorta (388 ± 87 [SE] vs 262 ± 56), placental artery (319 ± 95 vs 235 ± 54), and uterine artery (46 ± 6 vs 50 ± 13), values for fetal arteries exceeded those for uterine arteries (p ≤ 0.018) in spite of higher fetal plasma angiotensin II (74 ± 18 vs 30 ± 8 pg/ml, p < 0.01). Affinity did not differ between arteries and, except for fetal aorta (1.4 ± 0.2 vs 2.1 ± 0.4, r = 0.49, p = 0.045), was unchanged during late gestation. Conclusion: Differences between fetal and maternal responses and fetal placental and systemic responses to infused angiotensin II in sheep do not reflect alterations in total angiotensin II vascular smooth muscle receptor binding density or affinity.

Original languageEnglish (US)
Pages (from-to)1562-1569
Number of pages8
JournalAmerican Journal of Obstetrics and Gynecology
Volume168
Issue number5
StatePublished - 1993

Fingerprint

Uterine Artery
Vascular Smooth Muscle
Angiotensin II
Aorta
Sheep
Arteries
Mothers
Umbilicus
Pregnancy
Angiotensin Receptors
Iodine
Cell Membrane

Keywords

  • angiotensin II
  • fetus
  • pregnancy
  • receptors

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

@article{49efc38aa5ed4c9793a8dc3ffc01511e,
title = "Ontogeny of angiotensin II vascular smooth muscle receptors in ovine fetal aorta and placental and uterine arteries",
abstract = "Objective: Our purpose was to determine if differences in angiotensin II vascular smooth muscle receptor binding account for the attenuated fetal placental and systemic responses to infused angiotensin II relative to maternal responses and for the differences between umbilical and fetal systemic responses. Study design: Using plasma membranes prepared from the medial layer of fetal aorta, fetal placental artery, and maternal uterine artery obtained between 107 and 134 days of ovine gestation (n = 17), we measured and compared angiotensin II receptor binding density (in femtomoles per milligram of protein) and affinity (in nanomoles per liter) in radioligand binding studies with iodine 125-angiotensin II. Maternal and fetal plasma angiotensin II were also compared. Results: A single class of high-affinity receptor was identified in all arteries. Although the binding density was similar at < 100 and > 130 days for fetal aorta (388 ± 87 [SE] vs 262 ± 56), placental artery (319 ± 95 vs 235 ± 54), and uterine artery (46 ± 6 vs 50 ± 13), values for fetal arteries exceeded those for uterine arteries (p ≤ 0.018) in spite of higher fetal plasma angiotensin II (74 ± 18 vs 30 ± 8 pg/ml, p < 0.01). Affinity did not differ between arteries and, except for fetal aorta (1.4 ± 0.2 vs 2.1 ± 0.4, r = 0.49, p = 0.045), was unchanged during late gestation. Conclusion: Differences between fetal and maternal responses and fetal placental and systemic responses to infused angiotensin II in sheep do not reflect alterations in total angiotensin II vascular smooth muscle receptor binding density or affinity.",
keywords = "angiotensin II, fetus, pregnancy, receptors",
author = "Rosenfeld, {C. R.} and Cox, {B. E.} and Magness, {R. R.} and Shaul, {P. W.}",
year = "1993",
language = "English (US)",
volume = "168",
pages = "1562--1569",
journal = "American Journal of Obstetrics and Gynecology",
issn = "0002-9378",
publisher = "Mosby Inc.",
number = "5",

}

TY - JOUR

T1 - Ontogeny of angiotensin II vascular smooth muscle receptors in ovine fetal aorta and placental and uterine arteries

AU - Rosenfeld, C. R.

AU - Cox, B. E.

AU - Magness, R. R.

AU - Shaul, P. W.

PY - 1993

Y1 - 1993

N2 - Objective: Our purpose was to determine if differences in angiotensin II vascular smooth muscle receptor binding account for the attenuated fetal placental and systemic responses to infused angiotensin II relative to maternal responses and for the differences between umbilical and fetal systemic responses. Study design: Using plasma membranes prepared from the medial layer of fetal aorta, fetal placental artery, and maternal uterine artery obtained between 107 and 134 days of ovine gestation (n = 17), we measured and compared angiotensin II receptor binding density (in femtomoles per milligram of protein) and affinity (in nanomoles per liter) in radioligand binding studies with iodine 125-angiotensin II. Maternal and fetal plasma angiotensin II were also compared. Results: A single class of high-affinity receptor was identified in all arteries. Although the binding density was similar at < 100 and > 130 days for fetal aorta (388 ± 87 [SE] vs 262 ± 56), placental artery (319 ± 95 vs 235 ± 54), and uterine artery (46 ± 6 vs 50 ± 13), values for fetal arteries exceeded those for uterine arteries (p ≤ 0.018) in spite of higher fetal plasma angiotensin II (74 ± 18 vs 30 ± 8 pg/ml, p < 0.01). Affinity did not differ between arteries and, except for fetal aorta (1.4 ± 0.2 vs 2.1 ± 0.4, r = 0.49, p = 0.045), was unchanged during late gestation. Conclusion: Differences between fetal and maternal responses and fetal placental and systemic responses to infused angiotensin II in sheep do not reflect alterations in total angiotensin II vascular smooth muscle receptor binding density or affinity.

AB - Objective: Our purpose was to determine if differences in angiotensin II vascular smooth muscle receptor binding account for the attenuated fetal placental and systemic responses to infused angiotensin II relative to maternal responses and for the differences between umbilical and fetal systemic responses. Study design: Using plasma membranes prepared from the medial layer of fetal aorta, fetal placental artery, and maternal uterine artery obtained between 107 and 134 days of ovine gestation (n = 17), we measured and compared angiotensin II receptor binding density (in femtomoles per milligram of protein) and affinity (in nanomoles per liter) in radioligand binding studies with iodine 125-angiotensin II. Maternal and fetal plasma angiotensin II were also compared. Results: A single class of high-affinity receptor was identified in all arteries. Although the binding density was similar at < 100 and > 130 days for fetal aorta (388 ± 87 [SE] vs 262 ± 56), placental artery (319 ± 95 vs 235 ± 54), and uterine artery (46 ± 6 vs 50 ± 13), values for fetal arteries exceeded those for uterine arteries (p ≤ 0.018) in spite of higher fetal plasma angiotensin II (74 ± 18 vs 30 ± 8 pg/ml, p < 0.01). Affinity did not differ between arteries and, except for fetal aorta (1.4 ± 0.2 vs 2.1 ± 0.4, r = 0.49, p = 0.045), was unchanged during late gestation. Conclusion: Differences between fetal and maternal responses and fetal placental and systemic responses to infused angiotensin II in sheep do not reflect alterations in total angiotensin II vascular smooth muscle receptor binding density or affinity.

KW - angiotensin II

KW - fetus

KW - pregnancy

KW - receptors

UR - http://www.scopus.com/inward/record.url?scp=0027297650&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027297650&partnerID=8YFLogxK

M3 - Article

C2 - 8498444

AN - SCOPUS:0027297650

VL - 168

SP - 1562

EP - 1569

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

SN - 0002-9378

IS - 5

ER -