Opioid receptors in midbrain dopaminergic regions of the rat II. Kappa and delta receptor autoradiography

S. G. Speciale, K. F. Manaye, M. Sadeq, D. C. German

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Opiates and opioid peptides are known to influence the dopaminergic (DA) neurons in the midbrain. The purpose of this study was to map and quantify the density of kappa and delta opioid receptor subtypes in the retrorubral field, substantia nigra, and ventral tegmental area and related nuclei, which contain DA nuclei A8, A9, and A10, respectively. Sections through the rostral-caudal extent of the rat midbrain were stained with an antibody against tyrosine hydroxylase, as a DA cell marker, and comparable sections were processed for in vitro receptor autoradiography using the kappa-selective ligand, U-69593, and the delta-selective ligand, D-Pen2, D-Pen5-enkephalin. In general, both kappa and delta ligands exhibited low levels of specific binding in regions occupied by the midbrain DA neurons. Kappa binding (4-8 fmol/mg tissue) was high throughout the rostral-caudal extent of the substantia nigra, in rostral portions of the ventral tegmental area, and in the nucleus paranigralis; low binding occurred in the retrorubral field and central linear nucleus raphe. Delta binding (6-18 fmol/mg tissue) was high in the caudal portion of the substantia nigra pars reticulata, and in the medial terminal nucleus of the accessory optic system (a region previously shown to contain DA dendrites). The kappa and delta receptor binding is heterogeneously distributed in regions occupied by midbrain dopaminergic neurons, and several fold lower than the binding of mu opioid receptors in the same brain regions.

Original languageEnglish (US)
Pages (from-to)53-66
Number of pages14
JournalJournal of Neural Transmission
Volume91
Issue number1
DOIs
StatePublished - Feb 1993

Fingerprint

kappa Opioid Receptor
delta Opioid Receptor
Opioid Receptors
Mesencephalon
Autoradiography
Dopaminergic Neurons
Ventral Tegmental Area
Opioid Peptides
Substantia Nigra
Ligands
D-Penicillamine (2,5)-Enkephalin
mu Opioid Receptor
antineoplaston A10
Tyrosine 3-Monooxygenase
Dendrites
Antibodies
Brain

Keywords

  • kappa and delta opioid receptors
  • Midbrain dopaminergic neurons
  • rat
  • receptor autoradiography

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Opioid receptors in midbrain dopaminergic regions of the rat II. Kappa and delta receptor autoradiography. / Speciale, S. G.; Manaye, K. F.; Sadeq, M.; German, D. C.

In: Journal of Neural Transmission, Vol. 91, No. 1, 02.1993, p. 53-66.

Research output: Contribution to journalArticle

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