Opposite-strand RNAs from the 5' flanking region of the mouse dihydrofolate reductase gene

P. J. Farnham, J. M. Abrams, R. T. Schimke

Research output: Contribution to journalArticle

57 Scopus citations

Abstract

We have characterized a transcription unit in the 5' flanking region of the mouse gene encoding dihydrofolate reductase (EC 1.5.1.3) that is oriented in the opposite direction to that of dihydrofolate reductase transcription. These opposite-strand RNAs are 180-240 nucleotides long, differing in length at their 5' ends. They are abundant in nuclear RNA and are not polyadenylylated. We suggest that the promoter region of the dihydrofolate reductase gene functions in a bidirectional manner to produce both these RNAs and dihydrofolate reductase mRNAs.

Original languageEnglish (US)
Pages (from-to)3978-3982
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number12
DOIs
StatePublished - 1985

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