Opposite-strand RNAs from the 5' flanking region of the mouse dihydrofolate reductase gene

P. J. Farnham, J. M. Abrams, R. T. Schimke

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

We have characterized a transcription unit in the 5' flanking region of the mouse gene encoding dihydrofolate reductase (EC 1.5.1.3) that is oriented in the opposite direction to that of dihydrofolate reductase transcription. These opposite-strand RNAs are 180-240 nucleotides long, differing in length at their 5' ends. They are abundant in nuclear RNA and are not polyadenylylated. We suggest that the promoter region of the dihydrofolate reductase gene functions in a bidirectional manner to produce both these RNAs and dihydrofolate reductase mRNAs.

Original languageEnglish (US)
Pages (from-to)3978-3982
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number12
StatePublished - 1985

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Tetrahydrofolate Dehydrogenase
5' Flanking Region
RNA
Genes
Nuclear RNA
Genetic Promoter Regions
Nucleotides
Messenger RNA

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Opposite-strand RNAs from the 5' flanking region of the mouse dihydrofolate reductase gene. / Farnham, P. J.; Abrams, J. M.; Schimke, R. T.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, No. 12, 1985, p. 3978-3982.

Research output: Contribution to journalArticle

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