Optical detection of three modes of endocytosis at hippocampal synapses

Natali L. Chanaday, Ege T. Kavalali

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Coupling of synaptic vesicle fusion and retrieval constitutes a core mechanism ensuring maintenance of presynaptic function. Recent studies using fast-freeze electron microscopy and capacitance measurements reported an ultrafast mode of endocytosis operating at physiological temperatures. Here, using rat hippocampal neurons, we optically monitored single synaptic vesicle endocytosis with high time resolution using the vesicular glutamate transporter, synaptophysin and the V0a1 subunit of the vacuolar ATPase as probes. In this setting, we could distinguish three components of retrieval operating at ultrafast (~150–250 ms, ~20% of events), fast (~5–12 s, ~40% of events) and ultraslow speeds (>20 s, ~40% of events). While increasing Ca2+ slowed the fast events, increasing temperature accelerated their time course. In contrast, the kinetics of ultrafast events were only mildly affected by these manipulations. These results suggest that synaptic vesicle proteins can be retrieved with ultrafast kinetics, although a majority of evoked fusion events are coupled to slower retrieval mechanisms.

Original languageEnglish (US)
Article numbere36097
JournaleLife
Volume7
DOIs
StatePublished - Apr 23 2018

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Synaptic Vesicles
Endocytosis
Synapses
Fusion reactions
Vesicular Glutamate Transport Proteins
Vacuolar Proton-Translocating ATPases
Synaptophysin
Kinetics
Capacitance measurement
Electron microscopy
Neurons
Rats
Temperature
Electron Microscopy
Maintenance
Electrons
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Optical detection of three modes of endocytosis at hippocampal synapses. / Chanaday, Natali L.; Kavalali, Ege T.

In: eLife, Vol. 7, e36097, 23.04.2018.

Research output: Contribution to journalArticle

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