Optical imaging with a cathepsin B activated probe for the enhanced detection of esophageal adenocarcinoma by dual channel fluorescent upper GI endoscopy

Peiman Habibollahi, Jose Luiz Figueiredo, Pedram Heidari, Austin M. Dulak, Yu Imamura, Adam J. Bass, Shuji Ogino, Andrew T. Chan, Umar Mahmood

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Despite significant advances in diagnosis and treatment, the prognosis of esophageal adenocar-cinoma remains poor highlighting the importance of early detection. Although white light (WL) upper endoscopy can be used for screening of the esophagus, it has limited sensitivity for early stage disease. Thus, development of new imaging technology to improve the diagnostic capabilities of upper GI endoscopy for early detection of esophageal adenocarcinoma is an important unmet need. The goal of this study was to develop a method for the detection of malignant lesions in the esophagus using WL upper endoscopy combined with near infrared (NIR) imaging with a protease activatable probe (Prosense750) selective for cathepsin B (CTSB). An orthotopic murine model for distal esophageal adenocarcinoma was generated through the implantation of OE-33 and OE-19 human esophageal adenocarcinoma lines in immunocompromised mice. The mice were imaged simultaneously for WL and NIR signal using a custom-built dual channel upper GI endo-scope. The presence of tumor was confirmed by histology and target to background ratios (TBR) were compared for both WL and NIR imaging. NIR imaging with ProSense750 significantly im-proved upon the TBRs of esophageal tumor foci, with a TBR of 3.64±0.14 and 4.50±0.11 for the OE-33 and OE-19 tumors respectively, compared to 0.88±0.04 and 0.81±0.02 TBR for WL im-aging. The combination of protease probes with novel imaging devices has the potential to improve esophageal tumor detection by fluorescently highlighting neoplastic regions.

Original languageEnglish (US)
Pages (from-to)227-234
Number of pages8
JournalTheranostics
Volume2
Issue number2
DOIs
StatePublished - 2012
Externally publishedYes

Keywords

  • Cathepsin B
  • Esophageal adenocarcinoma
  • Near infrared imaging
  • Prosense750

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

Fingerprint Dive into the research topics of 'Optical imaging with a cathepsin B activated probe for the enhanced detection of esophageal adenocarcinoma by dual channel fluorescent upper GI endoscopy'. Together they form a unique fingerprint.

  • Cite this