@article{95571a67f1ae48438929065e1d7a18f8,
title = "Optimized protocol for stable isotope tracing and steady-state metabolomics in mouse HER2+ breast cancer brain metastasis",
abstract = "Analyzing the metabolic dependencies of tumor cells is vital for cancer diagnosis and treatment. Here, we describe a protocol for 13C-stable glucose and glutamine isotope tracing in mice HER2+ breast cancer brain metastatic lesions. We describe how to inject cancer cells intracardially to generate brain metastatic lesions in mice. We then detail how to perform 13C-stable isotope infusion in mice with established brain metastasis. Finally, we outline steps for sample collection, processing for metabolite extraction, and analyzing mass spectrometry data. For complete details on the use and execution of this protocol, please refer to Parida et al. (2022).",
keywords = "Cancer, Cell Biology, Cell culture, Mass Spectrometry, Metabolism, Metabolomics",
author = "Parida, {Pravat Kumar} and Mauricio Marquez-Palencia and Kaushik, {Akash K.} and Kangsan Kim and Vidhya Nair and Jessica Sudderth and Hieu Vu and Lauren Zacharias and Ralph DeBerardinis and Srinivas Malladi",
note = "Funding Information: This work was supported by grants from CPRIT training grant ( RP210041 ) to P.K.P., NSF grant ( 2019281049 ) to M.M.P., CPRIT ( RR170003 ), ACS ( RSG-20-47-01 -CSM), and METAvivor ( GAA202106-0027 ) grants to S.M. We gratefully acknowledge the assistance of the preclinical radiation core facility supported by CPRIT; RP180770 for bioluminescence imaging. Illustrations created with BioRender.com . Funding Information: This work was supported by grants from CPRIT training grant (RP210041) to P.K.P. NSF grant (2019281049) to M.M.P. CPRIT (RR170003), ACS (RSG-20-47-01-CSM), and METAvivor (GAA202106-0027) grants to S.M. We gratefully acknowledge the assistance of the preclinical radiation core facility supported by CPRIT; RP180770 for bioluminescence imaging. Illustrations created with BioRender.com. Experimental design and conceptualization, P.K.P. and S.M.; methodology, P.K.P. M.M.P. A.K.K. K.K. V.N. J.S. H.V. L.J. and S.M.; writing ? original draft, P.K.P. and S.M.; writing ? review & editing, P.K.P. R.J.D. and S.M.; funding acquisition, S.M.; supervision, S.M. R.J.D. is a founder of Atavistik Biosciences and an advisor for Agios Pharmaceuticals, Nirogy Therapeutics, and Vida Ventures. Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = jun,
day = "17",
doi = "10.1016/j.xpro.2022.101345",
language = "English (US)",
volume = "3",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",
}