Optimizing the synthesis of red- to near-IR-emitting CdS-capped CdTe xSe1-x alloyed quantum dots for biomedical imaging

Wen Jiang, Anupam Singhal, Jianing Zheng, Chen Wang, Warren C.W. Chan

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

Advancements in biomedical imaging require the development of optical contrast agents at an emission region of low biological tissue absorbance, fluorescence, and scattering. This region occurs in the red to near-IR (>600 nm) wavelength window. Quantum dots (Qdots) are excellent candidates for such applications. However, there are major challenges with developing high optical quality far-red- to near-IR-emitting Qdots (i.e., poor reproducibility, low quantum yield, and lack of photostability). Our aim is to systematically study how to prepare alloyed CdTexSe1-x with these properties. We discovered that the precursor concentrations of Te-to-Se and growth time had major impacts on the Qdot's optical properties. We also learned that the capping of these alloyed Qdots were difficult with ZnS but feasible with CdS because of the ZnS's lattice mismatch with the CdTexSe1-x. These systematic and basic studies led to the optimization of synthetic parameters for preparing Qdots with high quantum yield (>30%), narrow fluorescence full width at half-maxima (<50%), and stability against photobleaching (>10 min under 100W Hg lamp excitation with a 1.4 numerical aperture 60 × objective) for biomedical imaging and detection. We further demonstrate the conjugation of biorecognition molecules onto the surface of these alloyed Qdots and characterize their use as contrast agents in multicolored and ultrasensitive imaging.

Original languageEnglish (US)
Pages (from-to)4845-4854
Number of pages10
JournalChemistry of Materials
Volume18
Issue number20
DOIs
StatePublished - Oct 3 2006
Externally publishedYes

Fingerprint

Semiconductor quantum dots
Imaging techniques
Quantum yield
Contrast Media
Fluorescence
Lattice mismatch
Full width at half maximum
Electric lamps
Optical properties
Scattering
Tissue
Wavelength
Molecules

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)
  • Materials Chemistry

Cite this

Optimizing the synthesis of red- to near-IR-emitting CdS-capped CdTe xSe1-x alloyed quantum dots for biomedical imaging. / Jiang, Wen; Singhal, Anupam; Zheng, Jianing; Wang, Chen; Chan, Warren C.W.

In: Chemistry of Materials, Vol. 18, No. 20, 03.10.2006, p. 4845-4854.

Research output: Contribution to journalArticle

Jiang, Wen ; Singhal, Anupam ; Zheng, Jianing ; Wang, Chen ; Chan, Warren C.W. / Optimizing the synthesis of red- to near-IR-emitting CdS-capped CdTe xSe1-x alloyed quantum dots for biomedical imaging. In: Chemistry of Materials. 2006 ; Vol. 18, No. 20. pp. 4845-4854.
@article{7169a25bd8df46738f11e1a88db711b1,
title = "Optimizing the synthesis of red- to near-IR-emitting CdS-capped CdTe xSe1-x alloyed quantum dots for biomedical imaging",
abstract = "Advancements in biomedical imaging require the development of optical contrast agents at an emission region of low biological tissue absorbance, fluorescence, and scattering. This region occurs in the red to near-IR (>600 nm) wavelength window. Quantum dots (Qdots) are excellent candidates for such applications. However, there are major challenges with developing high optical quality far-red- to near-IR-emitting Qdots (i.e., poor reproducibility, low quantum yield, and lack of photostability). Our aim is to systematically study how to prepare alloyed CdTexSe1-x with these properties. We discovered that the precursor concentrations of Te-to-Se and growth time had major impacts on the Qdot's optical properties. We also learned that the capping of these alloyed Qdots were difficult with ZnS but feasible with CdS because of the ZnS's lattice mismatch with the CdTexSe1-x. These systematic and basic studies led to the optimization of synthetic parameters for preparing Qdots with high quantum yield (>30{\%}), narrow fluorescence full width at half-maxima (<50{\%}), and stability against photobleaching (>10 min under 100W Hg lamp excitation with a 1.4 numerical aperture 60 × objective) for biomedical imaging and detection. We further demonstrate the conjugation of biorecognition molecules onto the surface of these alloyed Qdots and characterize their use as contrast agents in multicolored and ultrasensitive imaging.",
author = "Wen Jiang and Anupam Singhal and Jianing Zheng and Chen Wang and Chan, {Warren C.W.}",
year = "2006",
month = "10",
day = "3",
doi = "10.1021/cm061311x",
language = "English (US)",
volume = "18",
pages = "4845--4854",
journal = "Chemistry of Materials",
issn = "0897-4756",
publisher = "American Chemical Society",
number = "20",

}

TY - JOUR

T1 - Optimizing the synthesis of red- to near-IR-emitting CdS-capped CdTe xSe1-x alloyed quantum dots for biomedical imaging

AU - Jiang, Wen

AU - Singhal, Anupam

AU - Zheng, Jianing

AU - Wang, Chen

AU - Chan, Warren C.W.

PY - 2006/10/3

Y1 - 2006/10/3

N2 - Advancements in biomedical imaging require the development of optical contrast agents at an emission region of low biological tissue absorbance, fluorescence, and scattering. This region occurs in the red to near-IR (>600 nm) wavelength window. Quantum dots (Qdots) are excellent candidates for such applications. However, there are major challenges with developing high optical quality far-red- to near-IR-emitting Qdots (i.e., poor reproducibility, low quantum yield, and lack of photostability). Our aim is to systematically study how to prepare alloyed CdTexSe1-x with these properties. We discovered that the precursor concentrations of Te-to-Se and growth time had major impacts on the Qdot's optical properties. We also learned that the capping of these alloyed Qdots were difficult with ZnS but feasible with CdS because of the ZnS's lattice mismatch with the CdTexSe1-x. These systematic and basic studies led to the optimization of synthetic parameters for preparing Qdots with high quantum yield (>30%), narrow fluorescence full width at half-maxima (<50%), and stability against photobleaching (>10 min under 100W Hg lamp excitation with a 1.4 numerical aperture 60 × objective) for biomedical imaging and detection. We further demonstrate the conjugation of biorecognition molecules onto the surface of these alloyed Qdots and characterize their use as contrast agents in multicolored and ultrasensitive imaging.

AB - Advancements in biomedical imaging require the development of optical contrast agents at an emission region of low biological tissue absorbance, fluorescence, and scattering. This region occurs in the red to near-IR (>600 nm) wavelength window. Quantum dots (Qdots) are excellent candidates for such applications. However, there are major challenges with developing high optical quality far-red- to near-IR-emitting Qdots (i.e., poor reproducibility, low quantum yield, and lack of photostability). Our aim is to systematically study how to prepare alloyed CdTexSe1-x with these properties. We discovered that the precursor concentrations of Te-to-Se and growth time had major impacts on the Qdot's optical properties. We also learned that the capping of these alloyed Qdots were difficult with ZnS but feasible with CdS because of the ZnS's lattice mismatch with the CdTexSe1-x. These systematic and basic studies led to the optimization of synthetic parameters for preparing Qdots with high quantum yield (>30%), narrow fluorescence full width at half-maxima (<50%), and stability against photobleaching (>10 min under 100W Hg lamp excitation with a 1.4 numerical aperture 60 × objective) for biomedical imaging and detection. We further demonstrate the conjugation of biorecognition molecules onto the surface of these alloyed Qdots and characterize their use as contrast agents in multicolored and ultrasensitive imaging.

UR - http://www.scopus.com/inward/record.url?scp=33750351927&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750351927&partnerID=8YFLogxK

U2 - 10.1021/cm061311x

DO - 10.1021/cm061311x

M3 - Article

AN - SCOPUS:33750351927

VL - 18

SP - 4845

EP - 4854

JO - Chemistry of Materials

JF - Chemistry of Materials

SN - 0897-4756

IS - 20

ER -