Oral supplementation of L-arginine prevents chronic cyclosporine nephrotoxicity in rats

Chul Woo Yang, Yong Soo Kim, Jin Kim, Young Ok Kim, So Youn Min, Euy Jin Choi, Byung Kee Bang

Research output: Contribution to journalArticle

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Abstract

This study was performed to evaluate the effect of L-arginine (L-Arg) on the prevention of chronic cyclosporine (CsA) nephrotoxicity in rats. Rats pair-fed a low-salt diet (0.05%) were given CsA (15 mg/kg/day s.c.), CsA and L-Arg (L-Arg group, 1.25 g/l water), CsA and N-nitro-L-arginine methyl ester (L-NAME group, 70 mg/l water) or vehicle. After 28 days, the L-Arg group had a higher glomerular filtration rate compared to the CsA (0.42 ± 0.05 vs. 0.31 ± 0.06 ml/min/100 g, p < 0.05) and the L-NAME groups (vs. 0.19 ± 0.04 ml/min/100 g, p < 0.05) and a significantly lower serum creatinine level compared to the CsA (0.70 ± 0.06 vs. 0.92 ± 0.12 mg/dl, p < 0.05) and the L-NAME groups (vs. 1.21 ± 0.17 mg/dl, p < 0.05). The L-Arg group had less fibrosis, tubular injury (TI), and arteriolopathy than the CsA (fibrosis 0.39 ± 0.14 vs. 0.74 ± 0.15; TI 1.3 ± 0.3 vs. 2.0 ± 0.1; arteriolopathy 33 ± 7 vs. 48 ± 17, p < 0.05, respectively) and the L-NAME groups (fibrosis vs. 1.67 ± 0.32, TI vs. 2.6 ± 0.3, arteriolopathy vs. 63 ± 10, p < 0.05, respectively). Plasma renin activity in the L-Arg group was less than in the CsA (18 ± 2 vs. 23 ± 3 ng Ang I/ml/h, p < 0.05) and the L-NAME groups (vs. 30 ± 3 ng Ang I/ml/h, p < 0.05). Nitric oxide production in L-Arg group was higher than in the CsA (24.2 ± 1.7 vs. 11.1 ± 1.5 μmol/24 h, p < 0.05) and the L-NAME groups (vs. 8.4 ± 1.0 μmol/24 h, p < 0.05). In conclusion, the nitric oxide pathway is associated with the pathogenesis of chronic CsA nephrotoxicity, and exogenous L-Arg supplementation is effective in reducing chronic CsA nephrotoxicity in rats.

Original languageEnglish (US)
Pages (from-to)50-56
Number of pages7
JournalExperimental Nephrology
Volume6
Issue number1
StatePublished - 1998

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NG-Nitroarginine Methyl Ester
Cyclosporine
Arginine
Fibrosis
Wounds and Injuries
Nitric Oxide
Sodium-Restricted Diet
Water
Glomerular Filtration Rate
Renin
Creatinine
Serum

Keywords

  • Cyclosporine
  • L-Arginine
  • N-Nitro-L-arginine methyl ester
  • Nitric oxide
  • Plasma renin activity

ASJC Scopus subject areas

  • Nephrology

Cite this

Yang, C. W., Kim, Y. S., Kim, J., Kim, Y. O., Min, S. Y., Choi, E. J., & Bang, B. K. (1998). Oral supplementation of L-arginine prevents chronic cyclosporine nephrotoxicity in rats. Experimental Nephrology, 6(1), 50-56.

Oral supplementation of L-arginine prevents chronic cyclosporine nephrotoxicity in rats. / Yang, Chul Woo; Kim, Yong Soo; Kim, Jin; Kim, Young Ok; Min, So Youn; Choi, Euy Jin; Bang, Byung Kee.

In: Experimental Nephrology, Vol. 6, No. 1, 1998, p. 50-56.

Research output: Contribution to journalArticle

Yang, CW, Kim, YS, Kim, J, Kim, YO, Min, SY, Choi, EJ & Bang, BK 1998, 'Oral supplementation of L-arginine prevents chronic cyclosporine nephrotoxicity in rats', Experimental Nephrology, vol. 6, no. 1, pp. 50-56.
Yang, Chul Woo ; Kim, Yong Soo ; Kim, Jin ; Kim, Young Ok ; Min, So Youn ; Choi, Euy Jin ; Bang, Byung Kee. / Oral supplementation of L-arginine prevents chronic cyclosporine nephrotoxicity in rats. In: Experimental Nephrology. 1998 ; Vol. 6, No. 1. pp. 50-56.
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title = "Oral supplementation of L-arginine prevents chronic cyclosporine nephrotoxicity in rats",
abstract = "This study was performed to evaluate the effect of L-arginine (L-Arg) on the prevention of chronic cyclosporine (CsA) nephrotoxicity in rats. Rats pair-fed a low-salt diet (0.05{\%}) were given CsA (15 mg/kg/day s.c.), CsA and L-Arg (L-Arg group, 1.25 g/l water), CsA and N-nitro-L-arginine methyl ester (L-NAME group, 70 mg/l water) or vehicle. After 28 days, the L-Arg group had a higher glomerular filtration rate compared to the CsA (0.42 ± 0.05 vs. 0.31 ± 0.06 ml/min/100 g, p < 0.05) and the L-NAME groups (vs. 0.19 ± 0.04 ml/min/100 g, p < 0.05) and a significantly lower serum creatinine level compared to the CsA (0.70 ± 0.06 vs. 0.92 ± 0.12 mg/dl, p < 0.05) and the L-NAME groups (vs. 1.21 ± 0.17 mg/dl, p < 0.05). The L-Arg group had less fibrosis, tubular injury (TI), and arteriolopathy than the CsA (fibrosis 0.39 ± 0.14 vs. 0.74 ± 0.15; TI 1.3 ± 0.3 vs. 2.0 ± 0.1; arteriolopathy 33 ± 7 vs. 48 ± 17, p < 0.05, respectively) and the L-NAME groups (fibrosis vs. 1.67 ± 0.32, TI vs. 2.6 ± 0.3, arteriolopathy vs. 63 ± 10, p < 0.05, respectively). Plasma renin activity in the L-Arg group was less than in the CsA (18 ± 2 vs. 23 ± 3 ng Ang I/ml/h, p < 0.05) and the L-NAME groups (vs. 30 ± 3 ng Ang I/ml/h, p < 0.05). Nitric oxide production in L-Arg group was higher than in the CsA (24.2 ± 1.7 vs. 11.1 ± 1.5 μmol/24 h, p < 0.05) and the L-NAME groups (vs. 8.4 ± 1.0 μmol/24 h, p < 0.05). In conclusion, the nitric oxide pathway is associated with the pathogenesis of chronic CsA nephrotoxicity, and exogenous L-Arg supplementation is effective in reducing chronic CsA nephrotoxicity in rats.",
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T1 - Oral supplementation of L-arginine prevents chronic cyclosporine nephrotoxicity in rats

AU - Yang, Chul Woo

AU - Kim, Yong Soo

AU - Kim, Jin

AU - Kim, Young Ok

AU - Min, So Youn

AU - Choi, Euy Jin

AU - Bang, Byung Kee

PY - 1998

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N2 - This study was performed to evaluate the effect of L-arginine (L-Arg) on the prevention of chronic cyclosporine (CsA) nephrotoxicity in rats. Rats pair-fed a low-salt diet (0.05%) were given CsA (15 mg/kg/day s.c.), CsA and L-Arg (L-Arg group, 1.25 g/l water), CsA and N-nitro-L-arginine methyl ester (L-NAME group, 70 mg/l water) or vehicle. After 28 days, the L-Arg group had a higher glomerular filtration rate compared to the CsA (0.42 ± 0.05 vs. 0.31 ± 0.06 ml/min/100 g, p < 0.05) and the L-NAME groups (vs. 0.19 ± 0.04 ml/min/100 g, p < 0.05) and a significantly lower serum creatinine level compared to the CsA (0.70 ± 0.06 vs. 0.92 ± 0.12 mg/dl, p < 0.05) and the L-NAME groups (vs. 1.21 ± 0.17 mg/dl, p < 0.05). The L-Arg group had less fibrosis, tubular injury (TI), and arteriolopathy than the CsA (fibrosis 0.39 ± 0.14 vs. 0.74 ± 0.15; TI 1.3 ± 0.3 vs. 2.0 ± 0.1; arteriolopathy 33 ± 7 vs. 48 ± 17, p < 0.05, respectively) and the L-NAME groups (fibrosis vs. 1.67 ± 0.32, TI vs. 2.6 ± 0.3, arteriolopathy vs. 63 ± 10, p < 0.05, respectively). Plasma renin activity in the L-Arg group was less than in the CsA (18 ± 2 vs. 23 ± 3 ng Ang I/ml/h, p < 0.05) and the L-NAME groups (vs. 30 ± 3 ng Ang I/ml/h, p < 0.05). Nitric oxide production in L-Arg group was higher than in the CsA (24.2 ± 1.7 vs. 11.1 ± 1.5 μmol/24 h, p < 0.05) and the L-NAME groups (vs. 8.4 ± 1.0 μmol/24 h, p < 0.05). In conclusion, the nitric oxide pathway is associated with the pathogenesis of chronic CsA nephrotoxicity, and exogenous L-Arg supplementation is effective in reducing chronic CsA nephrotoxicity in rats.

AB - This study was performed to evaluate the effect of L-arginine (L-Arg) on the prevention of chronic cyclosporine (CsA) nephrotoxicity in rats. Rats pair-fed a low-salt diet (0.05%) were given CsA (15 mg/kg/day s.c.), CsA and L-Arg (L-Arg group, 1.25 g/l water), CsA and N-nitro-L-arginine methyl ester (L-NAME group, 70 mg/l water) or vehicle. After 28 days, the L-Arg group had a higher glomerular filtration rate compared to the CsA (0.42 ± 0.05 vs. 0.31 ± 0.06 ml/min/100 g, p < 0.05) and the L-NAME groups (vs. 0.19 ± 0.04 ml/min/100 g, p < 0.05) and a significantly lower serum creatinine level compared to the CsA (0.70 ± 0.06 vs. 0.92 ± 0.12 mg/dl, p < 0.05) and the L-NAME groups (vs. 1.21 ± 0.17 mg/dl, p < 0.05). The L-Arg group had less fibrosis, tubular injury (TI), and arteriolopathy than the CsA (fibrosis 0.39 ± 0.14 vs. 0.74 ± 0.15; TI 1.3 ± 0.3 vs. 2.0 ± 0.1; arteriolopathy 33 ± 7 vs. 48 ± 17, p < 0.05, respectively) and the L-NAME groups (fibrosis vs. 1.67 ± 0.32, TI vs. 2.6 ± 0.3, arteriolopathy vs. 63 ± 10, p < 0.05, respectively). Plasma renin activity in the L-Arg group was less than in the CsA (18 ± 2 vs. 23 ± 3 ng Ang I/ml/h, p < 0.05) and the L-NAME groups (vs. 30 ± 3 ng Ang I/ml/h, p < 0.05). Nitric oxide production in L-Arg group was higher than in the CsA (24.2 ± 1.7 vs. 11.1 ± 1.5 μmol/24 h, p < 0.05) and the L-NAME groups (vs. 8.4 ± 1.0 μmol/24 h, p < 0.05). In conclusion, the nitric oxide pathway is associated with the pathogenesis of chronic CsA nephrotoxicity, and exogenous L-Arg supplementation is effective in reducing chronic CsA nephrotoxicity in rats.

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KW - L-Arginine

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KW - Nitric oxide

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