TY - JOUR
T1 - Organization and expression of the rat kallikrein gene family
AU - Wines, D. R.
AU - Brady, J. M.
AU - Pritchett, D. B.
AU - Roberts, J. L.
AU - MacDonald, R. J.
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1989
Y1 - 1989
N2 - We have cloned and characterized eight members of the kallikrein gene family from rat genomic DNA. Three of the cloned genes correspond to the previously characterized kallikrein family mRNAs PS, S2, and P1, which encode true kallikrein, tonin, and a novel kallikrein-like enzyme, respectively. In two cases, two kallikrein family genes are located on single genomic clones, suggesting close linkage of this family in the rat genome. Based on the total number of cloned genes and mRNAs, the minimum size of the rat family is 11 genes. Comparisons between the rat genes demonstrate a high degree of nucleotide sequence identity (>80%) in exonic, intronic, and nearby flanking regions. This extensive sequence conservation not limited to clearly functional domains suggests that concerted evolution for this gene family has occurred. Despite the high overall sequence conservation among the rat family members, several discrete regions of high variability exist in the coding regions. We have defined the tissue-specific expression of the PS (true kallikrein), S2 (tonin), and S3 mRNAs with gene-specific oligonucleotide probes derived from these variant regions. PS is expressed in a wide range of tissues, while S2 mRNA is restricted to the submaxillary gland and S3 mRNA to the submaxillary and prostate glands. The high sequence conservation within the upstream flanking regions of these genes suggests that a small number of nucleotide differences determines the disparate transcriptional specificity of individual family members.
AB - We have cloned and characterized eight members of the kallikrein gene family from rat genomic DNA. Three of the cloned genes correspond to the previously characterized kallikrein family mRNAs PS, S2, and P1, which encode true kallikrein, tonin, and a novel kallikrein-like enzyme, respectively. In two cases, two kallikrein family genes are located on single genomic clones, suggesting close linkage of this family in the rat genome. Based on the total number of cloned genes and mRNAs, the minimum size of the rat family is 11 genes. Comparisons between the rat genes demonstrate a high degree of nucleotide sequence identity (>80%) in exonic, intronic, and nearby flanking regions. This extensive sequence conservation not limited to clearly functional domains suggests that concerted evolution for this gene family has occurred. Despite the high overall sequence conservation among the rat family members, several discrete regions of high variability exist in the coding regions. We have defined the tissue-specific expression of the PS (true kallikrein), S2 (tonin), and S3 mRNAs with gene-specific oligonucleotide probes derived from these variant regions. PS is expressed in a wide range of tissues, while S2 mRNA is restricted to the submaxillary gland and S3 mRNA to the submaxillary and prostate glands. The high sequence conservation within the upstream flanking regions of these genes suggests that a small number of nucleotide differences determines the disparate transcriptional specificity of individual family members.
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M3 - Article
C2 - 2708383
AN - SCOPUS:0024516889
SN - 0021-9258
VL - 264
SP - 7653
EP - 7662
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 13
ER -