Organization of the 5' region of the rat ATP citrate lyase gene

K. S. Kim, S. W. Park, Y. A. Moon, Y. S. Kim

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

A genomic clone, encompassing the 5' flanking region and the first seven exons of rat ATP citrate lyase gene, was isolated from a rat genomic library and sequenced. Primer-extension analysis showed that mRNA is transcribed at 4407 nucleotides upstream from the translation start site. Primer-extension analysis and sequencing of ATP citrate lyase cDNA amplified by PCR showed that the promoter used for transcription is identical in mammary gland, lung, liver, brain and kidney. Southern-blot analysis showed that the ATP citrate lyase gene exists as a single copy. The 5' flanking region contains several consensus sequences defined as promoter elements. These include a CAAT box and Sp1-binding sites. However, a TATA box lacks this promoter. The expression of the chloramphenicol acetyltransferase gene was induced by the 5' flanking region (-2370 to -1) in the CHO cell line. The 5' flanking region also contains several sequence elements that may be involved in the transcriptional regulation of the gene.

Original languageEnglish (US)
Pages (from-to)759-764
Number of pages6
JournalBiochemical Journal
Volume302
Issue number3
DOIs
StatePublished - Jan 1 1994

    Fingerprint

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Kim, K. S., Park, S. W., Moon, Y. A., & Kim, Y. S. (1994). Organization of the 5' region of the rat ATP citrate lyase gene. Biochemical Journal, 302(3), 759-764. https://doi.org/10.1042/bj3020759