Organization of the 5' region of the rat ATP citrate lyase gene

K. S. Kim, S. W. Park, Y. A. Moon, Y. S. Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A genomic clone, encompassing the 5' flanking region and the first seven exons of rat ATP citrate lyase gene, was isolated from a rat genomic library and sequenced. Primer-extension analysis showed that mRNA is transcribed at 4407 nucleotides upstream from the translation start site. Primer-extension analysis and sequencing of ATP citrate lyase cDNA amplified by PCR showed that the promoter used for transcription is identical in mammary gland, lung, liver, brain and kidney. Southern-blot analysis showed that the ATP citrate lyase gene exists as a single copy. The 5' flanking region contains several consensus sequences defined as promoter elements. These include a CAAT box and Sp1-binding sites. However, a TATA box lacks this promoter. The expression of the chloramphenicol acetyltransferase gene was induced by the 5' flanking region (-2370 to -1) in the CHO cell line. The 5' flanking region also contains several sequence elements that may be involved in the transcriptional regulation of the gene.

Original languageEnglish (US)
Pages (from-to)759-764
Number of pages6
JournalBiochemical Journal
Volume302
Issue number3
StatePublished - 1994

Fingerprint

ATP Citrate (pro-S)-Lyase
5' Flanking Region
Rats
Genes
TATA Box
Chloramphenicol O-Acetyltransferase
Genomic Library
CHO Cells
Consensus Sequence
Transcription
Human Mammary Glands
Southern Blotting
Liver
Exons
Brain
Nucleotides
Complementary DNA
Clone Cells
Binding Sites
Kidney

ASJC Scopus subject areas

  • Biochemistry

Cite this

Kim, K. S., Park, S. W., Moon, Y. A., & Kim, Y. S. (1994). Organization of the 5' region of the rat ATP citrate lyase gene. Biochemical Journal, 302(3), 759-764.

Organization of the 5' region of the rat ATP citrate lyase gene. / Kim, K. S.; Park, S. W.; Moon, Y. A.; Kim, Y. S.

In: Biochemical Journal, Vol. 302, No. 3, 1994, p. 759-764.

Research output: Contribution to journalArticle

Kim, KS, Park, SW, Moon, YA & Kim, YS 1994, 'Organization of the 5' region of the rat ATP citrate lyase gene', Biochemical Journal, vol. 302, no. 3, pp. 759-764.
Kim KS, Park SW, Moon YA, Kim YS. Organization of the 5' region of the rat ATP citrate lyase gene. Biochemical Journal. 1994;302(3):759-764.
Kim, K. S. ; Park, S. W. ; Moon, Y. A. ; Kim, Y. S. / Organization of the 5' region of the rat ATP citrate lyase gene. In: Biochemical Journal. 1994 ; Vol. 302, No. 3. pp. 759-764.
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