Organoid Co-culture Methods to Capture Cancer Cell–Natural Killer Cell Interactions

Isaac S. Chan, Andrew J. Ewald

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Metastasis is a complex process that has been historically difficult to model in culture. Host immune responses play critical roles in restraining and promoting metastatic tumor cells. Here we describe a method of 3D organotypic co-culture of natural killer cells and tumor organoids to capture interactions between the two cellular populations. These assays can be used to model key aspects of metastatic biology and to screen for the effectiveness of agents that stimulate natural killer cell cytotoxicity.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages235-250
Number of pages16
DOIs
StatePublished - 2022
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2463
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • 3D culture
  • Antibody-dependent cell-mediated cytotoxicity
  • Breast cancer
  • Cancer metastasis
  • Immune escape
  • Innate immune system
  • Natural killer cells
  • Organoids
  • Organotypic co-culture
  • Tumor immunosurveillance

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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