@inbook{f605b78af379493fa897243b8fa870b3,
title = "Organoid Co-culture Methods to Capture Cancer Cell–Natural Killer Cell Interactions",
abstract = "Metastasis is a complex process that has been historically difficult to model in culture. Host immune responses play critical roles in restraining and promoting metastatic tumor cells. Here we describe a method of 3D organotypic co-culture of natural killer cells and tumor organoids to capture interactions between the two cellular populations. These assays can be used to model key aspects of metastatic biology and to screen for the effectiveness of agents that stimulate natural killer cell cytotoxicity.",
keywords = "3D culture, Antibody-dependent cell-mediated cytotoxicity, Breast cancer, Cancer metastasis, Immune escape, Innate immune system, Natural killer cells, Organoids, Organotypic co-culture, Tumor immunosurveillance",
author = "Chan, {Isaac S.} and Ewald, {Andrew J.}",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2160-8_17",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "235--250",
booktitle = "Methods in Molecular Biology",
}