Orientation of GST-tagged lectins via in situ surface modification to create an expanded lectin microarray for glycomic analysis

Daniel C. Propheter; Lara K. Mahal

doi:10.1039/c1mb05047h

Orientation of GST-tagged lectins via in situ surface modification to create an expanded lectin microarray for glycomic analysis

Daniel C. Propheter, Lara K. Mahal

Research output: Contribution to journal › Review article › peer-review

14 Scopus citations

Abstract

Herein we describe the orientation of GST-tagged lectins on NHS-activated slides via a one-step deposition of the protein and a glutathione (GSH) scaffold. This technology overcomes the need for a premade GSH-surface to orient GST-tagged proteins, enabling us to rapidly expand the analytical capacity of lectin microarrays through addition of oriented lectins, while maintaining lectin diversity.

Original language	English (US)
Pages (from-to)	2114-2117
Number of pages	4
Journal	Molecular BioSystems
Volume	7
Issue number	7
DOIs	https://doi.org/10.1039/c1mb05047h
State	Published - Jul 1 2011

ASJC Scopus subject areas

Biotechnology
Molecular Biology

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10.1039/c1mb05047h

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AB - Herein we describe the orientation of GST-tagged lectins on NHS-activated slides via a one-step deposition of the protein and a glutathione (GSH) scaffold. This technology overcomes the need for a premade GSH-surface to orient GST-tagged proteins, enabling us to rapidly expand the analytical capacity of lectin microarrays through addition of oriented lectins, while maintaining lectin diversity.

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Orientation of GST-tagged lectins via in situ surface modification to create an expanded lectin microarray for glycomic analysis

Abstract

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