Orientation of GST-tagged lectins via in situ surface modification to create an expanded lectin microarray for glycomic analysis

Daniel C. Propheter, Lara K. Mahal

Research output: Contribution to journalReview article

12 Scopus citations

Abstract

Herein we describe the orientation of GST-tagged lectins on NHS-activated slides via a one-step deposition of the protein and a glutathione (GSH) scaffold. This technology overcomes the need for a premade GSH-surface to orient GST-tagged proteins, enabling us to rapidly expand the analytical capacity of lectin microarrays through addition of oriented lectins, while maintaining lectin diversity.

Original languageEnglish (US)
Pages (from-to)2114-2117
Number of pages4
JournalMolecular BioSystems
Volume7
Issue number7
DOIs
Publication statusPublished - Jul 1 2011

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ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

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