Origin and function of Thy-1+ dendritic epidermal cells in mice

P. R. Bergstresser, S. Sullivan, J. W. Streilein, R. E. Tigelaar

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The epidermis of normal mouse skin incorporates a newly-recognized population of dendritic cells which express relatively large amounts of the cell surface glycoprotein, Thy-1 antigen. These cells, termed Thy-1+dEC, are distinct from both epidermal Langerhans cells (LC) and melanocytes, and they populate cutaneous sites in surface densities which range to as high as 580 cells/mm2, approximately two-thirds that of LC. Studies of lethally irradiated mice which were reconstituted with semiallogeneic bone marrow cells and mice which received grafts of semiallogeneic skin have demonstrated that some, if not all, Thy-1+dEC are of bone marrow origin, and that they are capable of migrating into epidermis from a vascular source. Thy-1+dEC expressed both asialo GM1 and a cell surface determinant recognized by the monoclonal antibody 20-10-5S, further suggesting their functions will be included among those normally ascribed to lymphoreticular cells. Isolation of epidermal cells with the Fluorescence Activated Cell Sorter (FACS) was successful in producing relatively pure populations of Thy-1+dEC and LC. Such technological advances as this should facilitate testing several hypotheses concerning the ultimate function of these cells, including the possibilities that they are antigen-presenting cells which selectively activate down-regulating signals, T lymphocytes, natural killer (NK) cells, or natural suppressor (NS) cells.

Original languageEnglish (US)
JournalJournal of Investigative Dermatology
Volume85
Issue numberSUPPL. 1
StatePublished - 1985

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Langerhans Cells
Skin
Bone
Thy-1 Antigens
T-cells
Membrane Glycoproteins
Grafts
Fluorescence
Monoclonal Antibodies
Cells
Epidermis
Testing
Cell Separation
Melanocytes
Antigen-Presenting Cells
Natural Killer Cells
Bone Marrow Cells
Dendritic Cells
Population
Blood Vessels

ASJC Scopus subject areas

  • Dermatology

Cite this

Bergstresser, P. R., Sullivan, S., Streilein, J. W., & Tigelaar, R. E. (1985). Origin and function of Thy-1+ dendritic epidermal cells in mice. Journal of Investigative Dermatology, 85(SUPPL. 1).

Origin and function of Thy-1+ dendritic epidermal cells in mice. / Bergstresser, P. R.; Sullivan, S.; Streilein, J. W.; Tigelaar, R. E.

In: Journal of Investigative Dermatology, Vol. 85, No. SUPPL. 1, 1985.

Research output: Contribution to journalArticle

Bergstresser, PR, Sullivan, S, Streilein, JW & Tigelaar, RE 1985, 'Origin and function of Thy-1+ dendritic epidermal cells in mice', Journal of Investigative Dermatology, vol. 85, no. SUPPL. 1.
Bergstresser PR, Sullivan S, Streilein JW, Tigelaar RE. Origin and function of Thy-1+ dendritic epidermal cells in mice. Journal of Investigative Dermatology. 1985;85(SUPPL. 1).
Bergstresser, P. R. ; Sullivan, S. ; Streilein, J. W. ; Tigelaar, R. E. / Origin and function of Thy-1+ dendritic epidermal cells in mice. In: Journal of Investigative Dermatology. 1985 ; Vol. 85, No. SUPPL. 1.
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