TY - JOUR
T1 - Origin and function of Thy-1+ dendritic epidermal cells in mice
AU - Bergstresser, P. R.
AU - Sullivan, S.
AU - Streilein, J. W.
AU - Tigelaar, R. E.
N1 - Funding Information:
Work in several laboratories has been directed at identifying the biologic relevance of this unique dendritic cell which expresses relatively large amounts of the cell surface glycoprotein, Supported in part by United States Public Health Service Grants Al-17363, CA-09082, and by a grant from Mary Kay Cosmetics, Inc. *Dr. Tigelaar is the recipient of NIH Research Center Career Development Award AM-00940. Reprint requests to: Paul R. Bergstresser, M.D., Department of Dermatology, University of Texas Health Science Center, 5323 Harry Hines Boulevard, Dallas, Texas 75235. Abbreviations: FACS: fluorescence activated cell sorter LC: Langerhans cells NK: natural killer NS: natural suppressor Thy-l+dEC: dendritic epidermal cells which express Thy-l gen UVB: ultraviolet radiation from spectrum B
PY - 1985
Y1 - 1985
N2 - The epidermis of normal mouse skin incorporates a newly-recognized population of dendritic cells which express relatively large amounts of the cell surface glycoprotein, Thy-1 antigen. These cells, termed Thy-1+dEC, are distinct from both epidermal Langerhans cells (LC) and melanocytes, and they populate cutaneous sites in surface densities which range to as high as 580 cells/mm2, approximately two-thirds that of LC. Studies of lethally irradiated mice which were reconstituted with semiallogeneic bone marrow cells and mice which received grafts of semiallogeneic skin have demonstrated that some, if not all, Thy-1+dEC are of bone marrow origin, and that they are capable of migrating into epidermis from a vascular source. Thy-1+dEC expressed both asialo GM1 and a cell surface determinant recognized by the monoclonal antibody 20-10-5S, further suggesting their functions will be included among those normally ascribed to lymphoreticular cells. Isolation of epidermal cells with the Fluorescence Activated Cell Sorter (FACS) was successful in producing relatively pure populations of Thy-1+dEC and LC. Such technological advances as this should facilitate testing several hypotheses concerning the ultimate function of these cells, including the possibilities that they are antigen-presenting cells which selectively activate down-regulating signals, T lymphocytes, natural killer (NK) cells, or natural suppressor (NS) cells.
AB - The epidermis of normal mouse skin incorporates a newly-recognized population of dendritic cells which express relatively large amounts of the cell surface glycoprotein, Thy-1 antigen. These cells, termed Thy-1+dEC, are distinct from both epidermal Langerhans cells (LC) and melanocytes, and they populate cutaneous sites in surface densities which range to as high as 580 cells/mm2, approximately two-thirds that of LC. Studies of lethally irradiated mice which were reconstituted with semiallogeneic bone marrow cells and mice which received grafts of semiallogeneic skin have demonstrated that some, if not all, Thy-1+dEC are of bone marrow origin, and that they are capable of migrating into epidermis from a vascular source. Thy-1+dEC expressed both asialo GM1 and a cell surface determinant recognized by the monoclonal antibody 20-10-5S, further suggesting their functions will be included among those normally ascribed to lymphoreticular cells. Isolation of epidermal cells with the Fluorescence Activated Cell Sorter (FACS) was successful in producing relatively pure populations of Thy-1+dEC and LC. Such technological advances as this should facilitate testing several hypotheses concerning the ultimate function of these cells, including the possibilities that they are antigen-presenting cells which selectively activate down-regulating signals, T lymphocytes, natural killer (NK) cells, or natural suppressor (NS) cells.
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U2 - 10.1111/1523-1747.ep12275516
DO - 10.1111/1523-1747.ep12275516
M3 - Article
C2 - 2409184
AN - SCOPUS:0022352689
SN - 0022-202X
VL - 85
SP - S85-S90
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - SUPPL. 1
ER -