Orthotopic hind limb transplantation in the mouse

Georg J. Furtmüller, Byoungchol Oh, Johanna Grahammer, Cheng Hung Lin, Robert Sucher, Madeline L. Fryer, Giorgio Raimondi, W. P. Andrew Lee, Gerald Brandacher

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

In vivo animal model systems, and in particular mouse models, have evolved into powerful and versatile scientific tools indispensable to basic and translational research in the field of transplantation medicine. A vast array of reagents is available exclusively in this setting, including monoand polyclonal antibodies for both diagnostic and interventional applications. In addition, a vast number of genotyped, inbred, transgenic, and knock out strains allow detailed investigation of the individual contributions of humoral and cellular components to the complex interplay of an immune response and make the mouse the gold standard for immunological research. Vascularized Composite Allotransplantation (VCA) delineates a novel field of transplantation using allografts to replace “like with like” in patients suffering traumatic or congenital tissue loss. This surgical methodological protocol shows the use of a non-suture cuff technique for supermicrovascular anastomosis in an orthotopic mouse hind limb transplantation model. The model specifically allows for comparison between established paradigms in solid organ transplantation with a novel form of transplants consisting of various different tissue components. Uniquely, this model allows for the transplantation of a viable vascularized bone marrow compartment and niche that have the potential to exert a beneficial effect on the balance of immune acceptance and rejection. This technique provides a tool to investigate alloantigen recognition and allograft rejection and acceptance, as well as enables the pursuit of functional nerve regeneration studies to further advance this novel field of transplantation.

Original languageEnglish (US)
Article numbere53483
JournalJournal of Visualized Experiments
Volume2016
Issue number108
DOIs
StatePublished - Feb 12 2016
Externally publishedYes

Keywords

  • Hind limb transplantation
  • Immunology
  • Issue 108
  • Medicine
  • Mouse
  • Non-suture cuff technique
  • Vascularized composite allotransplantation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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  • Cite this

    Furtmüller, G. J., Oh, B., Grahammer, J., Lin, C. H., Sucher, R., Fryer, M. L., Raimondi, G., Andrew Lee, W. P., & Brandacher, G. (2016). Orthotopic hind limb transplantation in the mouse. Journal of Visualized Experiments, 2016(108), [e53483]. https://doi.org/10.3791/53483