Osmotic tolerance of rabbit corneal endothelium in tissue culture

Dale R. Meyer, James P McCulley

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The osmotic tolerance of rabbit corneal endothelial cultures was determined for a balanced salt solution and various pharmaceutical vehicles in an effort to determine the biocompatibility of the different formulations after 4-128 min exposures. Acute change was assessed by monitoring the release of radiolabeled nucleotides and corresponding changes in tissue morphology of the prelabeled cultures. Protracted damage was evaluated by re-examining the morphology of the same cultures after 24 hr along with quantitative measurements of residual 3H release, tissue loss, and concomitant changes in protein synthesis. Our testing results indicated that the permissive limit of a balanced salt solution was between 200 and 400 mOsm, which is comparable to the guidelines for native endothelium in situ established by organ perfusion studies. In contrast, the permissive limits for prototypes of intraocular vehicles were more restricted, since only isotonic formulations were relatively nontoxic. Topical vehicles, by comparison, were much more damaging, which probably contraindicates their intraocular usage. These data indicate that this type of bioassay can be used effectively for screening and drug development programs, and for establishing accurate guidelines for pharmaceutical formulations.

Original languageEnglish (US)
Pages (from-to)31-46
Number of pages16
JournalCutaneous and Ocular Toxicology
Volume11
Issue number1
DOIs
StatePublished - 1992

Fingerprint

Corneal Endothelium
Tissue culture
Pharmaceutical Vehicles
Salts
Guidelines
Tissue
Rabbits
Drug Compounding
Preclinical Drug Evaluations
Bioassay
Biocompatibility
Cell culture
Biological Assay
Pharmaceutical Preparations
Endothelium
Screening
Nucleotides
Perfusion
Monitoring
Testing

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Ophthalmology

Cite this

Osmotic tolerance of rabbit corneal endothelium in tissue culture. / Meyer, Dale R.; McCulley, James P.

In: Cutaneous and Ocular Toxicology, Vol. 11, No. 1, 1992, p. 31-46.

Research output: Contribution to journalArticle

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