Overexpression of arginine decarboxylase in transgenic plants

Daniel Burtin, Anthony J. Michael

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The activity of arginine decarboxylase (ADC), a key enzyme in plant polyamine biosynthesis, was manipulated in two generations of transgenic tobacco plants. Second-generation transgenic plants overexpressing an oat ADC cDNA contained high levels of oat ADC transcript relative to tobacco ADC, possessed elevated ADC enzyme activity and accumulated 10-20-fold more agmatine, the direct product of ADC. In the presence of high levels of the precursor agmatine, no increase in the levels of the polyamines putrescine, spermidine and spermine was detected in the transgenic plants. Similarly, the activities of ornithine decarboxylase and S-adenosylmethionine decarboxylase were unchanged. No diversion of polyamine metabolism into the hydroxycinnamic acid-polyamine conjugate pool or into the tobacco alkaloid nicotine was detected. Activity of the catabolic enzyme diamine oxidase was the same in transgenic and control plants. The elevated ADC activity and agmatine production were subjected to a metabolic/physical block preventing increased, i.e. deregulated, polyamine accumulation. Overaccumulation of agmatine in the transgenic plants did not affect morphological development.

Original languageEnglish (US)
Pages (from-to)331-337
Number of pages7
JournalBiochemical Journal
Volume325
Issue number2
StatePublished - Jul 15 1997

Fingerprint

Genetically Modified Plants
Agmatine
Polyamines
Tobacco
Enzymes
Adenosylmethionine Decarboxylase
Amine Oxidase (Copper-Containing)
Coumaric Acids
Ornithine Decarboxylase
Putrescine
Spermidine
Spermine
Biosynthesis
Enzyme activity
arginine decarboxylase
Nicotine
Alkaloids
Metabolism
Complementary DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Overexpression of arginine decarboxylase in transgenic plants. / Burtin, Daniel; Michael, Anthony J.

In: Biochemical Journal, Vol. 325, No. 2, 15.07.1997, p. 331-337.

Research output: Contribution to journalArticle

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