Overexpression of chemokine-like factor 2 promotes the proliferation and survival of C2C12 skeletal muscle cells

Donglan Xia, Xianting Li, Yaxin Lou, Wenling Han, Peiguo Ding, Yingmei Zhang, Chunhui Di, Quansheng Song, Dalong Ma

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Chemokine-like factor 1 (CKLF1) is a novel cytokine first cloned from U937 cells. It contains different splicing forms and has chemotactic effects on a wide spectrum of cells both in vitro and in vivo; it can also stimulate the regeneration of skeletal muscle cells in vivo, but the mechanism remains unclear. To probe the myogenesis function of CKLF2, which is the largest isoform of CKLFs, C2C12 murine myoblasts were stably transfected with human CKLF2 eukaryotic expression vector. Compared with control vector transfected C2C12 cells, CKLF2 overexpression causes accelerated myoblast proliferation as determined by cell counting and [3H]TdR incorporation assays. In addition, CKLF2 overexpression also promotes cell differentiation, which was determined by higher expression levels of myogenin, creatine kinase, myosin and the accelerated myoblast fusion. Further analysis also indicates that CKLF2 could activate the transcription activity of the bHLH/MyoD and MEF2 families. Finally, DNA synthesis and myotube formation could also be promoted by growing C2C12 cells in conditioned media from CKLF2-transfected cells. These findings strongly suggest a role for human CKLF2 in regulation of skeletal muscle myogenesis.

Original languageEnglish (US)
Pages (from-to)163-173
Number of pages11
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1591
Issue number1-3
DOIs
StatePublished - Aug 19 2002

Keywords

  • C2C12
  • Chemokine-like factor 2
  • Differentiation
  • Muscle regeneration
  • Proliferation
  • Skeletal muscle

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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