Overexpression of complexin in PC12 cells inhibits exocytosis by preventing SNARE complex recycling

Jingguo Liu, Ting Guo, Ju Wu, Xiaochen Bai, Qiang Zhou, Sen Fang Sui

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Complexin is an important protein that functions during Ca 2+-dependent neurotransmitter release. Substantial evidence supports that complexin performs its role through rapid interaction with SNARE complex with high affinity. However, α-SNAP/NSF, which can disassemble the cis-SNARE complex in the presence of MgATP, competes with complexin to bind to SNARE complex. In addition, injection of α-SNAP into chromaffin cells enhances the size of the readily releasable pool, and mutation disrupting the ATPase activity of NSF results in the accumulation of SNARE complex. Thus, whether high concentrations of complexin could result in a reverse result is unclear. In this paper, we demonstrate that when stably overexpressed in PC12 cells, high levels of complexin result in the accumulation of SNARE complex. This in turn leads to a reduction in the size of the readily releasable pool of large dense core vesicles. These results suggest that high levels of complexin seem to prevent SNARE complex recycling, presumably by displacing NSF and α-SNAP from SNARE complex.

Original languageEnglish (US)
Pages (from-to)439-444
Number of pages6
JournalBiochemistry (Moscow)
Volume72
Issue number4
DOIs
StatePublished - Apr 1 2007

Fingerprint

SNARE Proteins
PC12 Cells
Exocytosis
Recycling
N-Ethylmaleimide-Sensitive Proteins
Chromaffin Cells
Secretory Vesicles
Cell Size
Neurotransmitter Agents
Adenosine Triphosphatases
Adenosine Triphosphate
Mutation
Injections

Keywords

  • Complexin
  • Neurotransmitter release
  • Readily releasable pool
  • SNARE complex cycle

ASJC Scopus subject areas

  • Biochemistry

Cite this

Overexpression of complexin in PC12 cells inhibits exocytosis by preventing SNARE complex recycling. / Liu, Jingguo; Guo, Ting; Wu, Ju; Bai, Xiaochen; Zhou, Qiang; Sui, Sen Fang.

In: Biochemistry (Moscow), Vol. 72, No. 4, 01.04.2007, p. 439-444.

Research output: Contribution to journalArticle

Liu, Jingguo ; Guo, Ting ; Wu, Ju ; Bai, Xiaochen ; Zhou, Qiang ; Sui, Sen Fang. / Overexpression of complexin in PC12 cells inhibits exocytosis by preventing SNARE complex recycling. In: Biochemistry (Moscow). 2007 ; Vol. 72, No. 4. pp. 439-444.
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