Overexpression of leptin receptors in pancreatic islets of Zucker diabetic fatty rats restores GLUT-2, glucokinase, and glucose- stimulated insulin secretion

May-Yun Wang, K. Koyama, M. Shimabukuro, David J Mangelsdorf, C. B. Newgard, Roger H Unger

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Abstract

The high-K(m) glucose transporter, GLUT-2, and the high-K(m) hexokinase of β cells, glucokinase (GK), are required for glucose- stimulated insulin secretion (GSIS). GLUT-2 expression in β cells of Zucker diabetic fatty (ZDF) rats is profoundly reduced at the onset of β-cell dysfunction of diabetes. Because ZDF rats are homozygous for a mutation in their leptin receptor (OB-R) gene and are therefore leptin-insensitive, we expressed the wild-type OB.R gene in diabetic islets by infusing a recombinant adenovirus (AdCMV-OB-Rb) to determine whether this reversed the abnormalities. Leptin induced a rise in phosphorylated STAT3, indicating that the transferred wild- type OB-R was functional. GLUT-2 protein rose 17-fold in AdCMV-OB- Rb-treated ZDF islets without leptin, and leptin caused no further rise. GK protein rose 7-fold without and 12-fold with leptin. Preproinsulin mRNA increased 64% without leptin and rose no further with leptin, but leptin was required to restore GSIS. Clofibrate and 9-cis-retinoic acid, the partner ligands for binding to peroxisome proliferator-activator receptor α (PPARα) and retinoid X receptor, up-regulated GLUT-2 expression in islets of normal rats, but not in ZDF rats, in which PPARα is very low. Because the fat content of islets of diabetic ZDF rats remains high unless they are treated with leptin, it appears that restoration of GSIS requires normalization of intracellular nutrient homeostasis, whereas up-regulation of GLUT-2 and GK is leptin-independent, requiring only high expression of OB- Rb.

Original languageEnglish (US)
Pages (from-to)11921-11926
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number20
DOIs
StatePublished - Sep 29 1998

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Glucokinase
Leptin Receptors
Leptin
Islets of Langerhans
Insulin
Glucose
Peroxisome Proliferators
vpr Genes
Glucose Transporter Type 2
Retinoid X Receptors
Clofibrate
Hexokinase
Facilitative Glucose Transport Proteins
Adenoviridae
Homeostasis
Up-Regulation
Fats
Ligands

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

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title = "Overexpression of leptin receptors in pancreatic islets of Zucker diabetic fatty rats restores GLUT-2, glucokinase, and glucose- stimulated insulin secretion",
abstract = "The high-K(m) glucose transporter, GLUT-2, and the high-K(m) hexokinase of β cells, glucokinase (GK), are required for glucose- stimulated insulin secretion (GSIS). GLUT-2 expression in β cells of Zucker diabetic fatty (ZDF) rats is profoundly reduced at the onset of β-cell dysfunction of diabetes. Because ZDF rats are homozygous for a mutation in their leptin receptor (OB-R) gene and are therefore leptin-insensitive, we expressed the wild-type OB.R gene in diabetic islets by infusing a recombinant adenovirus (AdCMV-OB-Rb) to determine whether this reversed the abnormalities. Leptin induced a rise in phosphorylated STAT3, indicating that the transferred wild- type OB-R was functional. GLUT-2 protein rose 17-fold in AdCMV-OB- Rb-treated ZDF islets without leptin, and leptin caused no further rise. GK protein rose 7-fold without and 12-fold with leptin. Preproinsulin mRNA increased 64{\%} without leptin and rose no further with leptin, but leptin was required to restore GSIS. Clofibrate and 9-cis-retinoic acid, the partner ligands for binding to peroxisome proliferator-activator receptor α (PPARα) and retinoid X receptor, up-regulated GLUT-2 expression in islets of normal rats, but not in ZDF rats, in which PPARα is very low. Because the fat content of islets of diabetic ZDF rats remains high unless they are treated with leptin, it appears that restoration of GSIS requires normalization of intracellular nutrient homeostasis, whereas up-regulation of GLUT-2 and GK is leptin-independent, requiring only high expression of OB- Rb.",
author = "May-Yun Wang and K. Koyama and M. Shimabukuro and Mangelsdorf, {David J} and Newgard, {C. B.} and Unger, {Roger H}",
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T1 - Overexpression of leptin receptors in pancreatic islets of Zucker diabetic fatty rats restores GLUT-2, glucokinase, and glucose- stimulated insulin secretion

AU - Wang, May-Yun

AU - Koyama, K.

AU - Shimabukuro, M.

AU - Mangelsdorf, David J

AU - Newgard, C. B.

AU - Unger, Roger H

PY - 1998/9/29

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