Overexpression of the tinman-related genes XNkx-2.5 and XNkx-2.3 in Xenopus embryos results in myocardial hyperplasia

Ondine B. Cleaver, Kristin D. Patterson, Paul A. Kriegt

Research output: Contribution to journalArticle

154 Citations (Scopus)

Abstract

Drosophila tinman is an NK-class homeobox gene required for formation of the dorsal vessel, the insect equivalent of the vertebrate heart. Vertebrate sequences related to tinman, such as mouse Nkx-2.5, chicken cNkx-2.5, Xenopus XNkx-2.5 and XNkx-2.3 are expressed in cardiac precursors and in tissues involved in induction of cardiac mesoderm. Mice which lack a functional Nkx-2.5 gene die due to cardiac defects. To determine the role of tinman-related sequences in heart development, we have overexpressed both XNkx-2.3 and XNkx-2.5 in Xenopus laevis embryos. The resulting embryos are morphologically normal except that they have enlarged hearts. The enlarged heart phenotype is due to a thickening of the myocardium caused by an increase in the overall number of myocardial cells (hyperplasia). Neither ectopic nor precocious expression of cardiac differentiation markers is detectable in overexpressing embryos. These results suggest that both XNkx-2.3 and XNkx-2.5 are functional homologues of tinman, responsible for maintenance of the heart field.

Original languageEnglish (US)
Pages (from-to)3549-3556
Number of pages8
JournalDevelopment
Volume122
Issue number11
StatePublished - 1996

Fingerprint

Xenopus
Hyperplasia
Embryonic Structures
Cardiomegaly
Vertebrates
Genes
Homeobox Genes
Differentiation Antigens
Xenopus laevis
Mesoderm
Drosophila
Insects
Chickens
Myocardium
Cell Count
Maintenance
Phenotype

Keywords

  • Cardiac development
  • Heart field
  • Homeobox
  • Organogenesis
  • tinman
  • Xenopus
  • XNkx-2.5

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Overexpression of the tinman-related genes XNkx-2.5 and XNkx-2.3 in Xenopus embryos results in myocardial hyperplasia. / Cleaver, Ondine B.; Patterson, Kristin D.; Kriegt, Paul A.

In: Development, Vol. 122, No. 11, 1996, p. 3549-3556.

Research output: Contribution to journalArticle

@article{390a3bf7e90a45989fc0a5c358e1ec53,
title = "Overexpression of the tinman-related genes XNkx-2.5 and XNkx-2.3 in Xenopus embryos results in myocardial hyperplasia",
abstract = "Drosophila tinman is an NK-class homeobox gene required for formation of the dorsal vessel, the insect equivalent of the vertebrate heart. Vertebrate sequences related to tinman, such as mouse Nkx-2.5, chicken cNkx-2.5, Xenopus XNkx-2.5 and XNkx-2.3 are expressed in cardiac precursors and in tissues involved in induction of cardiac mesoderm. Mice which lack a functional Nkx-2.5 gene die due to cardiac defects. To determine the role of tinman-related sequences in heart development, we have overexpressed both XNkx-2.3 and XNkx-2.5 in Xenopus laevis embryos. The resulting embryos are morphologically normal except that they have enlarged hearts. The enlarged heart phenotype is due to a thickening of the myocardium caused by an increase in the overall number of myocardial cells (hyperplasia). Neither ectopic nor precocious expression of cardiac differentiation markers is detectable in overexpressing embryos. These results suggest that both XNkx-2.3 and XNkx-2.5 are functional homologues of tinman, responsible for maintenance of the heart field.",
keywords = "Cardiac development, Heart field, Homeobox, Organogenesis, tinman, Xenopus, XNkx-2.5",
author = "Cleaver, {Ondine B.} and Patterson, {Kristin D.} and Kriegt, {Paul A.}",
year = "1996",
language = "English (US)",
volume = "122",
pages = "3549--3556",
journal = "Development (Cambridge)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "11",

}

TY - JOUR

T1 - Overexpression of the tinman-related genes XNkx-2.5 and XNkx-2.3 in Xenopus embryos results in myocardial hyperplasia

AU - Cleaver, Ondine B.

AU - Patterson, Kristin D.

AU - Kriegt, Paul A.

PY - 1996

Y1 - 1996

N2 - Drosophila tinman is an NK-class homeobox gene required for formation of the dorsal vessel, the insect equivalent of the vertebrate heart. Vertebrate sequences related to tinman, such as mouse Nkx-2.5, chicken cNkx-2.5, Xenopus XNkx-2.5 and XNkx-2.3 are expressed in cardiac precursors and in tissues involved in induction of cardiac mesoderm. Mice which lack a functional Nkx-2.5 gene die due to cardiac defects. To determine the role of tinman-related sequences in heart development, we have overexpressed both XNkx-2.3 and XNkx-2.5 in Xenopus laevis embryos. The resulting embryos are morphologically normal except that they have enlarged hearts. The enlarged heart phenotype is due to a thickening of the myocardium caused by an increase in the overall number of myocardial cells (hyperplasia). Neither ectopic nor precocious expression of cardiac differentiation markers is detectable in overexpressing embryos. These results suggest that both XNkx-2.3 and XNkx-2.5 are functional homologues of tinman, responsible for maintenance of the heart field.

AB - Drosophila tinman is an NK-class homeobox gene required for formation of the dorsal vessel, the insect equivalent of the vertebrate heart. Vertebrate sequences related to tinman, such as mouse Nkx-2.5, chicken cNkx-2.5, Xenopus XNkx-2.5 and XNkx-2.3 are expressed in cardiac precursors and in tissues involved in induction of cardiac mesoderm. Mice which lack a functional Nkx-2.5 gene die due to cardiac defects. To determine the role of tinman-related sequences in heart development, we have overexpressed both XNkx-2.3 and XNkx-2.5 in Xenopus laevis embryos. The resulting embryos are morphologically normal except that they have enlarged hearts. The enlarged heart phenotype is due to a thickening of the myocardium caused by an increase in the overall number of myocardial cells (hyperplasia). Neither ectopic nor precocious expression of cardiac differentiation markers is detectable in overexpressing embryos. These results suggest that both XNkx-2.3 and XNkx-2.5 are functional homologues of tinman, responsible for maintenance of the heart field.

KW - Cardiac development

KW - Heart field

KW - Homeobox

KW - Organogenesis

KW - tinman

KW - Xenopus

KW - XNkx-2.5

UR - http://www.scopus.com/inward/record.url?scp=0029955134&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029955134&partnerID=8YFLogxK

M3 - Article

VL - 122

SP - 3549

EP - 3556

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

IS - 11

ER -