Overproduction of valuable methoxylated flavones in induced tetraploid plants of Dracocephalum kotschyi Boiss

Ali Akbar Zahedi, Bahman Hosseini, Mohammad Fattahi, Esmail Dehghan, Hadi Parastar, Hadi Madani

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Background: Ploidy manipulation is considered an efficient method to increase production potential of medicinally important compounds. Dracocephalum kotschyi Boiss. is an endangered medicinal plant of Iran. Various concentrations of colchicine (0.05, 0.10, 0.20, and 0.50% w/v) were applied to shoot apical meristems of D. kotschyi seedlings in two and four-leaf stages to induce tetraploidy. Results: According to the results, 0.5% (w/v) of colchicine can be effective for polyploidy induction in D. kotschyi. Putative tetraploids were selected by morphological and microscopic characteristics and their ploidy level was confirmed by flow cytometry analysis and chromosome counting. The chromosome number of original diploid plant was confirmed to be 2n = 2× = 20 whereas that of the tetraploid plant was 2n = 4× = 40. Tetraploid and mixoploid plants showed different morphological, physiological and microscopic characteristics from those of diploid counterparts. The total content of flavonoids was increased from 1583.28 in diploids to 1890.07 (μg/g DW) in stable tetraploids. Conclusion: High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) confirmed over accumulation of methoxylated hydroxyflavones in solid tetraploid plants of D. kotschyi.

Original languageEnglish (US)
JournalBotanical Studies
Volume55
Issue number1
DOIs
StatePublished - Feb 4 2014
Externally publishedYes

Keywords

  • Chromosome counting
  • Dracocephalum kotschyi Boiss
  • Flow cytometry
  • HPLC-DAD
  • Tetraploidy
  • Xanthomicrol

ASJC Scopus subject areas

  • Plant Science

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