Ovine fetoplacental sulfoconjugation and aromatization of dehydroepiandrosterone

C. R. Rosenfeld, R. J. Worley, L. Milewich, N. F. Grant, C. R. Parker

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Infusions of dehydroepiandrosterone (D) into pregnant ewes result in increased maternal plasma concentrations of estrone and estradiol. To investigate the role of the ovine fetoplacental unit in the conversion of D to estrogen, 6 mg D were infused into the fetal vena cava in seven chronically instrumented pregnant ewes between 124-150 days gestation. Simultaneous serial blood samples were obtained from the umbilical artery and vein, the uterine vein, and the iliac artery (representative of the uterine artery) and assayed for estrone, estradiol, D and D sulfate. The concentration of D in the umbilical artery increased from 1.35±0.35 ng/ml at time zero to 49±15 ng/ml at 15 min (P<0.025) and remained elevated at 30 min. D was unchanged in samples from the umbilical vein and uterine artery and vein. The umbilical arteriovenous difference was significant (P<0.05). Plasma estrone and estradiol increased significantly (P<0.05) in both fetal and maternal compartments 7.5 min after the fetal administration of D; fetal values rose higher than maternal values. Maximum concentrations of D sulfate were also achieved at 7.5 min; fetal values remained greater than maternal values (P<0.001), and significant uterine and umbilical arteriovenous differences were observed during the first 30 min (P<0.05). It is evident from these in vivo studies that D is extracted from umbilical cord blood by the fetal placenta, that estrogens are promptly synthesized after the infusion of D into the fetus, that sulfokinase activity in the fetoplacental unit is great, and that ovine fetoplacental aromatase activity is not rate limiting in the conversion of D to estrogen after the 124th day of pregnancy.

Original languageEnglish (US)
Pages (from-to)1971-1979
Number of pages9
JournalEndocrinology
Volume106
Issue number6
StatePublished - 1980

Fingerprint

Dehydroepiandrosterone
Umbilical Arteries
Estrone
Sheep
Mothers
Umbilicus
Estradiol
Uterine Artery
Umbilical Veins
Estrogens
Sulfates
Veins
Arylsulfotransferase
Pregnancy
Venae Cavae
Aromatase
Iliac Artery
Fetal Blood
Placenta
Fetus

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Rosenfeld, C. R., Worley, R. J., Milewich, L., Grant, N. F., & Parker, C. R. (1980). Ovine fetoplacental sulfoconjugation and aromatization of dehydroepiandrosterone. Endocrinology, 106(6), 1971-1979.

Ovine fetoplacental sulfoconjugation and aromatization of dehydroepiandrosterone. / Rosenfeld, C. R.; Worley, R. J.; Milewich, L.; Grant, N. F.; Parker, C. R.

In: Endocrinology, Vol. 106, No. 6, 1980, p. 1971-1979.

Research output: Contribution to journalArticle

Rosenfeld, CR, Worley, RJ, Milewich, L, Grant, NF & Parker, CR 1980, 'Ovine fetoplacental sulfoconjugation and aromatization of dehydroepiandrosterone', Endocrinology, vol. 106, no. 6, pp. 1971-1979.
Rosenfeld CR, Worley RJ, Milewich L, Grant NF, Parker CR. Ovine fetoplacental sulfoconjugation and aromatization of dehydroepiandrosterone. Endocrinology. 1980;106(6):1971-1979.
Rosenfeld, C. R. ; Worley, R. J. ; Milewich, L. ; Grant, N. F. ; Parker, C. R. / Ovine fetoplacental sulfoconjugation and aromatization of dehydroepiandrosterone. In: Endocrinology. 1980 ; Vol. 106, No. 6. pp. 1971-1979.
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