Oxidative stress in cancer associated fibroblasts drives tumor-stroma co-evolution

A new paradigm for understanding tumor metabolism, the field effect and genomic instability in cancer cells

Ubaldo E. Martinez-Outschoorn, Renee M. Balliet, Dayana B. Rivadeneira, Barbara Chiavarina, Stephanos Pavlides, Chenguang Wang, Diana Whitaker-Menezes, Kristin M. Daumer, Zhao Lin, Agnieszka K. Witkiewicz, Neal Flomenberg, Anthony Howell, Richard G. Pestell, Erik S. Knudsen, Federica Sotgia, Michael P. Lisanti

Research output: Contribution to journalArticle

283 Citations (Scopus)

Abstract

Loss of stromal fibroblast caveolin-1 (Cav-1) is a powerful single independent predictor of poor prognosis in human breast cancer patients, and is associated with early tumor recurrence, lymph node metastasis and tamoxifen-resistance. We developed a novel co-culture system to understand the mechanism(s) by which a loss of stromal fibroblast Cav-1 induces a "lethal tumor micro-environment." Here, we propose a new paradigm to explain the powerful prognostic value of stromal Cav-1. In this model, cancer cells induce oxidative stress in cancer-associated fibroblasts, which then acts as a "metabolic" and "mutagenic" motor to drive tumor-stroma co-evolution, DNA damage and aneuploidy in cancer cells. More specifically, we show that an acute loss of Cav-1 expression leads to mitochondrial dysfunction, oxidative stress and aerobic glycolysis in cancer associated fibroblasts. Also, we propose that defective mitochondria are removed from cancer-associated fibroblasts by autophagy/mitophagy that is induced by oxidative stress. As a consequence, cancer associated fibroblasts provide nutrients (such as lactate) to stimulate mitochondrial biogenesis and oxidative metabolism in adjacent cancer cells (the "Reverse Warburg Effect"). We provide evidence that oxidative stress in cancer-associated fibroblasts is sufficient to induce genomic instability in adjacent cancer cells, via a bystander effect, potentially increasing their aggressive behavior. Finally, we directly demonstrate that nitric oxide (NO) over-production, secondary to Cav-1 loss, is the root cause for mitochondrial dysfunction in cancer associated fibroblasts. In support of this notion, treatment with anti-oxidants (such as N-acetyl-cysteine, metformin and quercetin) or NO inhibitors (L-NAME) was sufficient to reverse many of the cancer-associated fibroblast phenotypes that we describe. Thus, cancer cells use "oxidative stress" in adjacent fibroblasts (i) as an "engine" to fuel their own survival via the stromal production of nutrients and (ii) to drive their own mutagenic evolution towards a more aggressive phenotype, by promoting genomic instability. We also present evidence that the "field effect" in cancer biology could also be related to the stromal production of ROS and NO species. eNOS-expressing fibroblasts have the ability to downregulate Cav-1 and induce mitochondrial dysfunction in adjacent fibroblasts that do not express eNOS. As such, the effects of stromal oxidative stress can be laterally propagated, amplified and are effectively "contagious" - spread from cell-to-cell like a virus - creating an "oncogenic/mutagenic" field promoting widespread DNA damage.

Original languageEnglish (US)
Pages (from-to)3256-3276
Number of pages21
JournalCell Cycle
Volume9
Issue number16
DOIs
StatePublished - Aug 15 2010

Fingerprint

Genomic Instability
Caveolin 1
Oxidative Stress
Fibroblasts
Neoplasms
Nitric Oxide
DNA Damage
Mitochondrial Degradation
Bystander Effect
Phenotype
Cancer-Associated Fibroblasts
Food
Oncogenic Viruses
Aptitude
Metformin
NG-Nitroarginine Methyl Ester
Quercetin
Autophagy
Aneuploidy
Organelle Biogenesis

Keywords

  • Aneuploidy
  • Anti-oxidant cancer therapy
  • Autophagy
  • Cancer associated fibroblasts
  • Caveolin-1
  • DNA damage
  • Genomic instability
  • In cancer biology
  • Mitochondrial dysfunction
  • Nitric oxide (NO)
  • Oxidative stress
  • Reactive oxygen species (ROS)
  • The "field effect"

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Cite this

Oxidative stress in cancer associated fibroblasts drives tumor-stroma co-evolution : A new paradigm for understanding tumor metabolism, the field effect and genomic instability in cancer cells. / Martinez-Outschoorn, Ubaldo E.; Balliet, Renee M.; Rivadeneira, Dayana B.; Chiavarina, Barbara; Pavlides, Stephanos; Wang, Chenguang; Whitaker-Menezes, Diana; Daumer, Kristin M.; Lin, Zhao; Witkiewicz, Agnieszka K.; Flomenberg, Neal; Howell, Anthony; Pestell, Richard G.; Knudsen, Erik S.; Sotgia, Federica; Lisanti, Michael P.

In: Cell Cycle, Vol. 9, No. 16, 15.08.2010, p. 3256-3276.

Research output: Contribution to journalArticle

Martinez-Outschoorn, UE, Balliet, RM, Rivadeneira, DB, Chiavarina, B, Pavlides, S, Wang, C, Whitaker-Menezes, D, Daumer, KM, Lin, Z, Witkiewicz, AK, Flomenberg, N, Howell, A, Pestell, RG, Knudsen, ES, Sotgia, F & Lisanti, MP 2010, 'Oxidative stress in cancer associated fibroblasts drives tumor-stroma co-evolution: A new paradigm for understanding tumor metabolism, the field effect and genomic instability in cancer cells', Cell Cycle, vol. 9, no. 16, pp. 3256-3276. https://doi.org/10.4161/cc.9.16.12553
Martinez-Outschoorn, Ubaldo E. ; Balliet, Renee M. ; Rivadeneira, Dayana B. ; Chiavarina, Barbara ; Pavlides, Stephanos ; Wang, Chenguang ; Whitaker-Menezes, Diana ; Daumer, Kristin M. ; Lin, Zhao ; Witkiewicz, Agnieszka K. ; Flomenberg, Neal ; Howell, Anthony ; Pestell, Richard G. ; Knudsen, Erik S. ; Sotgia, Federica ; Lisanti, Michael P. / Oxidative stress in cancer associated fibroblasts drives tumor-stroma co-evolution : A new paradigm for understanding tumor metabolism, the field effect and genomic instability in cancer cells. In: Cell Cycle. 2010 ; Vol. 9, No. 16. pp. 3256-3276.
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T1 - Oxidative stress in cancer associated fibroblasts drives tumor-stroma co-evolution

T2 - A new paradigm for understanding tumor metabolism, the field effect and genomic instability in cancer cells

AU - Martinez-Outschoorn, Ubaldo E.

AU - Balliet, Renee M.

AU - Rivadeneira, Dayana B.

AU - Chiavarina, Barbara

AU - Pavlides, Stephanos

AU - Wang, Chenguang

AU - Whitaker-Menezes, Diana

AU - Daumer, Kristin M.

AU - Lin, Zhao

AU - Witkiewicz, Agnieszka K.

AU - Flomenberg, Neal

AU - Howell, Anthony

AU - Pestell, Richard G.

AU - Knudsen, Erik S.

AU - Sotgia, Federica

AU - Lisanti, Michael P.

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N2 - Loss of stromal fibroblast caveolin-1 (Cav-1) is a powerful single independent predictor of poor prognosis in human breast cancer patients, and is associated with early tumor recurrence, lymph node metastasis and tamoxifen-resistance. We developed a novel co-culture system to understand the mechanism(s) by which a loss of stromal fibroblast Cav-1 induces a "lethal tumor micro-environment." Here, we propose a new paradigm to explain the powerful prognostic value of stromal Cav-1. In this model, cancer cells induce oxidative stress in cancer-associated fibroblasts, which then acts as a "metabolic" and "mutagenic" motor to drive tumor-stroma co-evolution, DNA damage and aneuploidy in cancer cells. More specifically, we show that an acute loss of Cav-1 expression leads to mitochondrial dysfunction, oxidative stress and aerobic glycolysis in cancer associated fibroblasts. Also, we propose that defective mitochondria are removed from cancer-associated fibroblasts by autophagy/mitophagy that is induced by oxidative stress. As a consequence, cancer associated fibroblasts provide nutrients (such as lactate) to stimulate mitochondrial biogenesis and oxidative metabolism in adjacent cancer cells (the "Reverse Warburg Effect"). We provide evidence that oxidative stress in cancer-associated fibroblasts is sufficient to induce genomic instability in adjacent cancer cells, via a bystander effect, potentially increasing their aggressive behavior. Finally, we directly demonstrate that nitric oxide (NO) over-production, secondary to Cav-1 loss, is the root cause for mitochondrial dysfunction in cancer associated fibroblasts. In support of this notion, treatment with anti-oxidants (such as N-acetyl-cysteine, metformin and quercetin) or NO inhibitors (L-NAME) was sufficient to reverse many of the cancer-associated fibroblast phenotypes that we describe. Thus, cancer cells use "oxidative stress" in adjacent fibroblasts (i) as an "engine" to fuel their own survival via the stromal production of nutrients and (ii) to drive their own mutagenic evolution towards a more aggressive phenotype, by promoting genomic instability. We also present evidence that the "field effect" in cancer biology could also be related to the stromal production of ROS and NO species. eNOS-expressing fibroblasts have the ability to downregulate Cav-1 and induce mitochondrial dysfunction in adjacent fibroblasts that do not express eNOS. As such, the effects of stromal oxidative stress can be laterally propagated, amplified and are effectively "contagious" - spread from cell-to-cell like a virus - creating an "oncogenic/mutagenic" field promoting widespread DNA damage.

AB - Loss of stromal fibroblast caveolin-1 (Cav-1) is a powerful single independent predictor of poor prognosis in human breast cancer patients, and is associated with early tumor recurrence, lymph node metastasis and tamoxifen-resistance. We developed a novel co-culture system to understand the mechanism(s) by which a loss of stromal fibroblast Cav-1 induces a "lethal tumor micro-environment." Here, we propose a new paradigm to explain the powerful prognostic value of stromal Cav-1. In this model, cancer cells induce oxidative stress in cancer-associated fibroblasts, which then acts as a "metabolic" and "mutagenic" motor to drive tumor-stroma co-evolution, DNA damage and aneuploidy in cancer cells. More specifically, we show that an acute loss of Cav-1 expression leads to mitochondrial dysfunction, oxidative stress and aerobic glycolysis in cancer associated fibroblasts. Also, we propose that defective mitochondria are removed from cancer-associated fibroblasts by autophagy/mitophagy that is induced by oxidative stress. As a consequence, cancer associated fibroblasts provide nutrients (such as lactate) to stimulate mitochondrial biogenesis and oxidative metabolism in adjacent cancer cells (the "Reverse Warburg Effect"). We provide evidence that oxidative stress in cancer-associated fibroblasts is sufficient to induce genomic instability in adjacent cancer cells, via a bystander effect, potentially increasing their aggressive behavior. Finally, we directly demonstrate that nitric oxide (NO) over-production, secondary to Cav-1 loss, is the root cause for mitochondrial dysfunction in cancer associated fibroblasts. In support of this notion, treatment with anti-oxidants (such as N-acetyl-cysteine, metformin and quercetin) or NO inhibitors (L-NAME) was sufficient to reverse many of the cancer-associated fibroblast phenotypes that we describe. Thus, cancer cells use "oxidative stress" in adjacent fibroblasts (i) as an "engine" to fuel their own survival via the stromal production of nutrients and (ii) to drive their own mutagenic evolution towards a more aggressive phenotype, by promoting genomic instability. We also present evidence that the "field effect" in cancer biology could also be related to the stromal production of ROS and NO species. eNOS-expressing fibroblasts have the ability to downregulate Cav-1 and induce mitochondrial dysfunction in adjacent fibroblasts that do not express eNOS. As such, the effects of stromal oxidative stress can be laterally propagated, amplified and are effectively "contagious" - spread from cell-to-cell like a virus - creating an "oncogenic/mutagenic" field promoting widespread DNA damage.

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KW - Anti-oxidant cancer therapy

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KW - DNA damage

KW - Genomic instability

KW - In cancer biology

KW - Mitochondrial dysfunction

KW - Nitric oxide (NO)

KW - Oxidative stress

KW - Reactive oxygen species (ROS)

KW - The "field effect"

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