Oxidative stress-induced intestinal epithelial cell apoptosis is mediated by p38 MAPK

Yuning Zhou, Qingding Wang, B. Mark Evers, Dai H. Chung

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Free oxygen radicals are involved in the pathogenesis of necrotizing enterocolitis (NEC) in premature infants. The stress-activated p38 mitogen-activated protein kinase (MAPK) has been implicated in gut injury. Here, we found that phosphorylated p38 was detected primarily in the villus tips of normal intestine, whereas it was expressed in the entire mucosa in NEC. H2O2 treatment resulted in a rapid phosphorylation of p38 MAPK and subsequent apoptosis of rat intestinal epithelial (RIE)-1 cells; this induction was attenuated by treatment with SB203580, a selective p38 MAPK inhibitor, or transfection with p38α siRNA. Moreover, SB203580 also blocked H2O2-induced PKC activation. In contrast, the PKC inhibitor (GF109203x) did not affect p38 activation, indicating that p38 MAPK activation occurs upstream of PKC activation in H2O2-induced apoptosis. H2O2 treatment also decreased mitochondrial membrane potential; pretreatment with SB203580 attenuated this response. Our study demonstrates that the p38 MAPK/PKC pathway plays an important role as a pro-apoptotic cellular signaling during oxidative stress-induced intestinal epithelial cell injury.

Original languageEnglish (US)
Pages (from-to)860-865
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume350
Issue number4
DOIs
StatePublished - Dec 1 2006
Externally publishedYes

Fingerprint

Oxidative stress
p38 Mitogen-Activated Protein Kinases
Oxidative Stress
Epithelial Cells
Apoptosis
Chemical activation
Necrotizing Enterocolitis
Cell signaling
Phosphorylation
Mitochondrial Membrane Potential
Wounds and Injuries
Protein Kinase Inhibitors
Premature Infants
Small Interfering RNA
Free Radicals
Intestines
Transfection
Rats
Reactive Oxygen Species
Mucous Membrane

Keywords

  • Apoptosis
  • Intestinal epithelial cell injury
  • NEC
  • Oxidative stress
  • p38 MAPK

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Oxidative stress-induced intestinal epithelial cell apoptosis is mediated by p38 MAPK. / Zhou, Yuning; Wang, Qingding; Mark Evers, B.; Chung, Dai H.

In: Biochemical and Biophysical Research Communications, Vol. 350, No. 4, 01.12.2006, p. 860-865.

Research output: Contribution to journalArticle

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