Oxygen or glucose deprivation-induced neuronal injury in cortical cell cultures is reduced by tetanus toxin

H. Monyer, R. G. Giffard, D. M. Hartley, L. L. Dugan, M. P. Goldberg, D. W. Choi

Research output: Contribution to journalArticle

75 Scopus citations

Abstract

We examined glutamate-mediated neurotoxicity in cortical cell cultures pretreated with 1-5 μg/ml tetanus toxin to attenuate the Ca2+-dependent release of neurotransmitters. Efficacy of the tetanus toxin pretreatment was suggested by blockade of electrical burst activity induced by Mg2+ removal and by reduction of glutamate efflux induced by high K+. Tetanus toxin reduced neuronal injury produced by brief exposure to elevated extracellular K+ or to glutamate, situations in which release of endogenous excitatory neurotransmitter is likely to play a role. Furthermore, although glutamate efflux evoked by anoxic conditions may occur largely via Ca2+-independent transport, tetanus toxin attenuated both glutamate efflux and neuronal injury following combined oxygen and glucose deprivation. With prolonged exposure periods, the neuroprotective efficacy of tetanus toxin was comparable to that of NMDA receptor antagonists. Presynaptic inhibition of Ca 2+-dependent glutamate release may be a valuable approach to attenuating hypoxic-ischemic brain injury.

Original languageEnglish (US)
Pages (from-to)967-973
Number of pages7
JournalNeuron
Volume8
Issue number5
DOIs
StatePublished - May 1992

ASJC Scopus subject areas

  • Neuroscience(all)

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