Oxygen triggers signal transduction in the DevS (DosS) sensor of Mycobacterium tuberculosis by modulating the quaternary structure

Josiane Bezerra da Silva Lobão, Ana C.S. Gondim, Wellinson G. Guimarães, Marie-Alda Gilles-Gonzalez, Luiz Gonzaga de França Lopes, Eduardo H.S. Sousa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A major challenge to the control and eventual eradication of Mycobacterium tuberculosis infection is this pathogen's prolonged dormancy. The heme-based oxygen sensor protein DevS (DosS) plays a key role in this phenomenon, because it is a major activator of the transcription factor DevR. When DevS is active, its histidine protein kinase region is ON and it phosphorylates and activates DevR, which can induce the transcription of the dormancy regulon genes. Here, we have investigated the mechanism by which the ligation of molecular oxygen to a heme-binding domain in DevS switches OFF its histidine protein kinase region. To shed light on the oligomerization states of this protein and possible protein-surfaces of interaction, we used analytical gel filtration, together with dynamic light scattering, fluorescence spectroscopy and chemical crosslinking. We found that DevS exists as three major species: an octamer, a tetramer and a dimer. These three states were observed for the concentration range between 0.5 and 20 μm DevS, but not below 0.1 μm. Levels of DevS in M. tuberculosis are expected to range from 5 to 26 μm. When this histidine protein kinase was OFF, the DevS was mainly tetrameric and dimeric; by contrast, when the kinase was ON, the protein was predominantly octameric. The changes in quaternary structure were rapid upon binding to the physiological signal. This finding represents a novel strategy for switching the activity of a two-component heme-based sensor. An enhanced understanding of this process might potentially lead to the design of novel regulatory agents that target the multimer interfaces for treatment of latent tuberculosis.

Original languageEnglish (US)
JournalFEBS Journal
DOIs
StateAccepted/In press - Jan 1 2019

Fingerprint

Signal transduction
Heme
Mycobacterium tuberculosis
Histidine
Protein Kinases
Signal Transduction
Oxygen
Sensors
Latent Tuberculosis
Regulon
Oxygen sensors
Oligomerization
Mycobacterium Infections
Proteins
Molecular oxygen
Fluorescence Spectrometry
Fluorescence spectroscopy
Pathogens
Dynamic light scattering
Transcription

Keywords

  • DevS
  • heme-based sensor
  • signal transduction
  • tuberculosis
  • two-component system

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Oxygen triggers signal transduction in the DevS (DosS) sensor of Mycobacterium tuberculosis by modulating the quaternary structure. / Lobão, Josiane Bezerra da Silva; Gondim, Ana C.S.; Guimarães, Wellinson G.; Gilles-Gonzalez, Marie-Alda; Lopes, Luiz Gonzaga de França; Sousa, Eduardo H.S.

In: FEBS Journal, 01.01.2019.

Research output: Contribution to journalArticle

Lobão, Josiane Bezerra da Silva ; Gondim, Ana C.S. ; Guimarães, Wellinson G. ; Gilles-Gonzalez, Marie-Alda ; Lopes, Luiz Gonzaga de França ; Sousa, Eduardo H.S. / Oxygen triggers signal transduction in the DevS (DosS) sensor of Mycobacterium tuberculosis by modulating the quaternary structure. In: FEBS Journal. 2019.
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