Oxytocin stimulates glucagon and insulin secretion in fetal and neonatal sheep

Lawrence A. Wallin, C. Peter Fawcett, Charles R. Rosenfeld

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In adults of several species arginine vasopressin (AVP) and oxytocin (OT) stimulate pancreatic secretion of immunoreactive plasma glucagon (IRG). In fetal sheep AVP is an important stress hormone and may be simultaneously secreted with OT; however, their effects on IRG secretion are not known. We sought to determine if AVP and/or OT affected pancreatic IRG secretion in fetal and neonatal sheep. Either AVP or OT was infused for 30 min in chronically catheterized fetal and neonatal sheep, obtaining peripheral arterial and/or portal venous blood samples before; 10, 15, and 30 min during; and 15, 30, and 60 min after infusion for measurements of blood gases, hematocrit, IRG, immunoreactive plasma insulin (IRI) and plasma glucose. AVP did not affect IRG or IRI in fetal sheep (mean ± SE, 133 ± 1 days gestation), but small increases occurred in portal venous blood of lambs (2–49 days old). In contrast, OT (4.6 ± 0.3 mU/min · kg; n = 12) increased fetal plasma IRG from 72 ± 5 to 86 ± 6 and 97 ± 7 pg/ml (P < 0.001) and IRI from 16 ± 2 to 20 ± 3 and 20 ± 2 μU/ml (P < 0.02) at 15 and 30 min, respectively; 157 ± 11 μU OT/min–kg had no effect. In lambs (2–49 days old), 3.0 mU OT/min–kg increased arterial (n = 15) IRG from 139 ± 19 to 367 ± 43 and 483 ± 76 pg/ml (P < 0.01) and portal IRG (n = 8) from 167 ± 39 to 341 ± 72 and 502 ± 148 pg/ml (P < 0.01), respectively. Arterial and portal IRI also rose (P < 0.01) from 36 ± 4 to 82 ± 12 and 105 ± 32 μU/ml and from 29 ± 5 to 65 ± 13 and 51 ± 7 μU/ml, respectively. Glucose was unchanged in all experiments. In fetal and neonatal sheep, AVP has minimal effects on IRG and IRI release. In contrast, OT increases both substantially; furthermore, there is a difference in fetal and neonatal responsiveness. OT may be important in modulating glucagon and insulin secretion during and after parturition.

Original languageEnglish (US)
Pages (from-to)2235-2243
Number of pages9
JournalEndocrinology
Volume125
Issue number5
DOIs
StatePublished - 1989

Fingerprint

Oxytocin
Glucagon
Sheep
Insulin
Arginine Vasopressin
Vasotocin
Glucose
Hematocrit

ASJC Scopus subject areas

  • Endocrinology

Cite this

Oxytocin stimulates glucagon and insulin secretion in fetal and neonatal sheep. / Wallin, Lawrence A.; Fawcett, C. Peter; Rosenfeld, Charles R.

In: Endocrinology, Vol. 125, No. 5, 1989, p. 2235-2243.

Research output: Contribution to journalArticle

Wallin, Lawrence A. ; Fawcett, C. Peter ; Rosenfeld, Charles R. / Oxytocin stimulates glucagon and insulin secretion in fetal and neonatal sheep. In: Endocrinology. 1989 ; Vol. 125, No. 5. pp. 2235-2243.
@article{3d46989c258a4265be7dd0a496b863be,
title = "Oxytocin stimulates glucagon and insulin secretion in fetal and neonatal sheep",
abstract = "In adults of several species arginine vasopressin (AVP) and oxytocin (OT) stimulate pancreatic secretion of immunoreactive plasma glucagon (IRG). In fetal sheep AVP is an important stress hormone and may be simultaneously secreted with OT; however, their effects on IRG secretion are not known. We sought to determine if AVP and/or OT affected pancreatic IRG secretion in fetal and neonatal sheep. Either AVP or OT was infused for 30 min in chronically catheterized fetal and neonatal sheep, obtaining peripheral arterial and/or portal venous blood samples before; 10, 15, and 30 min during; and 15, 30, and 60 min after infusion for measurements of blood gases, hematocrit, IRG, immunoreactive plasma insulin (IRI) and plasma glucose. AVP did not affect IRG or IRI in fetal sheep (mean ± SE, 133 ± 1 days gestation), but small increases occurred in portal venous blood of lambs (2–49 days old). In contrast, OT (4.6 ± 0.3 mU/min · kg; n = 12) increased fetal plasma IRG from 72 ± 5 to 86 ± 6 and 97 ± 7 pg/ml (P < 0.001) and IRI from 16 ± 2 to 20 ± 3 and 20 ± 2 μU/ml (P < 0.02) at 15 and 30 min, respectively; 157 ± 11 μU OT/min–kg had no effect. In lambs (2–49 days old), 3.0 mU OT/min–kg increased arterial (n = 15) IRG from 139 ± 19 to 367 ± 43 and 483 ± 76 pg/ml (P < 0.01) and portal IRG (n = 8) from 167 ± 39 to 341 ± 72 and 502 ± 148 pg/ml (P < 0.01), respectively. Arterial and portal IRI also rose (P < 0.01) from 36 ± 4 to 82 ± 12 and 105 ± 32 μU/ml and from 29 ± 5 to 65 ± 13 and 51 ± 7 μU/ml, respectively. Glucose was unchanged in all experiments. In fetal and neonatal sheep, AVP has minimal effects on IRG and IRI release. In contrast, OT increases both substantially; furthermore, there is a difference in fetal and neonatal responsiveness. OT may be important in modulating glucagon and insulin secretion during and after parturition.",
author = "Wallin, {Lawrence A.} and Fawcett, {C. Peter} and Rosenfeld, {Charles R.}",
year = "1989",
doi = "10.1210/endo-125-5-2289",
language = "English (US)",
volume = "125",
pages = "2235--2243",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "5",

}

TY - JOUR

T1 - Oxytocin stimulates glucagon and insulin secretion in fetal and neonatal sheep

AU - Wallin, Lawrence A.

AU - Fawcett, C. Peter

AU - Rosenfeld, Charles R.

PY - 1989

Y1 - 1989

N2 - In adults of several species arginine vasopressin (AVP) and oxytocin (OT) stimulate pancreatic secretion of immunoreactive plasma glucagon (IRG). In fetal sheep AVP is an important stress hormone and may be simultaneously secreted with OT; however, their effects on IRG secretion are not known. We sought to determine if AVP and/or OT affected pancreatic IRG secretion in fetal and neonatal sheep. Either AVP or OT was infused for 30 min in chronically catheterized fetal and neonatal sheep, obtaining peripheral arterial and/or portal venous blood samples before; 10, 15, and 30 min during; and 15, 30, and 60 min after infusion for measurements of blood gases, hematocrit, IRG, immunoreactive plasma insulin (IRI) and plasma glucose. AVP did not affect IRG or IRI in fetal sheep (mean ± SE, 133 ± 1 days gestation), but small increases occurred in portal venous blood of lambs (2–49 days old). In contrast, OT (4.6 ± 0.3 mU/min · kg; n = 12) increased fetal plasma IRG from 72 ± 5 to 86 ± 6 and 97 ± 7 pg/ml (P < 0.001) and IRI from 16 ± 2 to 20 ± 3 and 20 ± 2 μU/ml (P < 0.02) at 15 and 30 min, respectively; 157 ± 11 μU OT/min–kg had no effect. In lambs (2–49 days old), 3.0 mU OT/min–kg increased arterial (n = 15) IRG from 139 ± 19 to 367 ± 43 and 483 ± 76 pg/ml (P < 0.01) and portal IRG (n = 8) from 167 ± 39 to 341 ± 72 and 502 ± 148 pg/ml (P < 0.01), respectively. Arterial and portal IRI also rose (P < 0.01) from 36 ± 4 to 82 ± 12 and 105 ± 32 μU/ml and from 29 ± 5 to 65 ± 13 and 51 ± 7 μU/ml, respectively. Glucose was unchanged in all experiments. In fetal and neonatal sheep, AVP has minimal effects on IRG and IRI release. In contrast, OT increases both substantially; furthermore, there is a difference in fetal and neonatal responsiveness. OT may be important in modulating glucagon and insulin secretion during and after parturition.

AB - In adults of several species arginine vasopressin (AVP) and oxytocin (OT) stimulate pancreatic secretion of immunoreactive plasma glucagon (IRG). In fetal sheep AVP is an important stress hormone and may be simultaneously secreted with OT; however, their effects on IRG secretion are not known. We sought to determine if AVP and/or OT affected pancreatic IRG secretion in fetal and neonatal sheep. Either AVP or OT was infused for 30 min in chronically catheterized fetal and neonatal sheep, obtaining peripheral arterial and/or portal venous blood samples before; 10, 15, and 30 min during; and 15, 30, and 60 min after infusion for measurements of blood gases, hematocrit, IRG, immunoreactive plasma insulin (IRI) and plasma glucose. AVP did not affect IRG or IRI in fetal sheep (mean ± SE, 133 ± 1 days gestation), but small increases occurred in portal venous blood of lambs (2–49 days old). In contrast, OT (4.6 ± 0.3 mU/min · kg; n = 12) increased fetal plasma IRG from 72 ± 5 to 86 ± 6 and 97 ± 7 pg/ml (P < 0.001) and IRI from 16 ± 2 to 20 ± 3 and 20 ± 2 μU/ml (P < 0.02) at 15 and 30 min, respectively; 157 ± 11 μU OT/min–kg had no effect. In lambs (2–49 days old), 3.0 mU OT/min–kg increased arterial (n = 15) IRG from 139 ± 19 to 367 ± 43 and 483 ± 76 pg/ml (P < 0.01) and portal IRG (n = 8) from 167 ± 39 to 341 ± 72 and 502 ± 148 pg/ml (P < 0.01), respectively. Arterial and portal IRI also rose (P < 0.01) from 36 ± 4 to 82 ± 12 and 105 ± 32 μU/ml and from 29 ± 5 to 65 ± 13 and 51 ± 7 μU/ml, respectively. Glucose was unchanged in all experiments. In fetal and neonatal sheep, AVP has minimal effects on IRG and IRI release. In contrast, OT increases both substantially; furthermore, there is a difference in fetal and neonatal responsiveness. OT may be important in modulating glucagon and insulin secretion during and after parturition.

UR - http://www.scopus.com/inward/record.url?scp=85003113606&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85003113606&partnerID=8YFLogxK

U2 - 10.1210/endo-125-5-2289

DO - 10.1210/endo-125-5-2289

M3 - Article

C2 - 2676481

AN - SCOPUS:85003113606

VL - 125

SP - 2235

EP - 2243

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 5

ER -