P16 as a molecular biomarker of cervical adenocarcinoma

John O. Schorge, Jayanthi S. Lea, Keren J. Elias, Ramababu Rajanbabu, Robert L. Coleman, David S. Miller, Raheela Ashfaq

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

Objective: Cervical adenocarcinomas are increasing in incidence each year. The aim of this study was to identify a molecular biomarker to improve early detection. Study design: Fifty-five in situ and invasive cervical adenocarcinomas were compared with 5 normal endocervical controls by immunohistochemical analysis of p16, p21, p27, cyclin D1, cyclin E, p53, and Ki-67. Expression was scored from 0 to 8 by using an automated imaging system. Western blotting and polymerase chain reaction-based human papillomavirus (HPV) testing were performed on 16 of the invasive cases having fresh-frozen tissue. Results: P16 exhibited a higher mean expression score for in situ (7.4; P < .0001) and invasive cervical adenocarcinoma (6.6; P < .0001) versus controls (2.0). A cutoff p16 expression score of 5 had a sensitivity of 94.5% and a specificity of 100%. Western blotting confirmed p16 protein expression. Fourteen (88%) of 16 invasive cervical adenocarcinomas were HPV-positive. Conclusion: P16 is a putative molecular biomarker of cervical adenocarcinoma. Overexpression appears to primarily reflect HPV-induced cell cycle dysregulation.

Original languageEnglish (US)
Pages (from-to)668-673
Number of pages6
JournalAmerican journal of obstetrics and gynecology
Volume190
Issue number3
DOIs
StatePublished - Mar 2004

Keywords

  • Adenocarcinoma in situ
  • Biomarker
  • Cervical adenocarcinoma
  • P16

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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