p172: An Alveolar Type II and Clara Cell Specific Protein with Late Developmental Expression and Upregulation by Hyperoxic Lung Injury

Carlos E. Girod, Dong Ho Shin, Marc B. Hershenson, Julian Solway, Rolf Dahl, York E. Miller

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The epithelium of the alveolus and distal airway meets unique requirements, functioning as a gas exchange membrane and barrier to alveolar flooding by vascular contents as well as to bloodstream contamination by airborne toxins and pathogens. Gene products specifically expressed by this epithelium, notably the surfactant apoproteins, have had important clinical application. No cell surface antigen specific for alveolar type II and Clara cells has been described. We report the biochemical characterization, tissue and developmental expression, and upregulation by injury of a 172 kD protein recognized by a monoclonal antibody, 3F9, synthesized in response to immunization with freshly isolated rat alveolar type II cells. p172 is expressed in a polarized fashion by the apical surface of rat alveolar type II and Clara cells. An immunohistochemical survey of various rat tissues and organs reveals lung specificity. p172 is first detectable in rare epithelial cells at 19 days of gestation, a time when the fully differentiated alveolar type II cell is identified by the first detection of lamellar bodies. There is a dramatic increase in p172 expression just prior to birth. Hyperoxic lung injury results in increased expression of p172. The upregulation of p172 by hyperoxia and its cell-specific expression suggests an important adaptive function.

Original languageEnglish (US)
Pages (from-to)538-547
Number of pages10
JournalAmerican journal of respiratory cell and molecular biology
Volume14
Issue number6
DOIs
StatePublished - 1996

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

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