p53 activity is selectively licensed in the Drosophila stem cell compartment

Annika Wylie, Wan Jin Lu, Alejandro D'Brot, Michael Buszczak, John M. Abrams

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Oncogenic stress provokes tumor suppression by p53 but the extent to which this regulatory axis is conserved remains unknown. Using a biosensor to visualize p53 action, we find that Drosophila p53 is selectively active in gonadal stem cells after exposure to stressors that destabilize the genome. Similar p53 activity occurred in hyperplastic growths that were triggered either by the RasV12 oncoprotein or by failed differentiation programs. In a model of transient sterility, p53 was required for the recovery of fertility after stress, and entry into the cell cycle was delayed in p53- stem cells. Together, these observations establish that the stem cell compartment of the Drosophila germline is selectively licensed for stress-induced activation of the p53 regulatory network. Furthermore, the findings uncover ancestral links between p53 and aberrant proliferation that are independent of DNA breaks and predate evolution of the ARF/Mdm2 axis.

Original languageEnglish (US)
Article numbere01530
JournaleLife
Volume2014
Issue number3
DOIs
StatePublished - Mar 11 2014

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Stem cells
Drosophila
Stem Cells
DNA Breaks
Oncogene Proteins
Biosensing Techniques
Prednisolone
Biosensors
Infertility
Fertility
Tumors
Cell Cycle
Genes
Chemical activation
Cells
Genome
Recovery
DNA
Growth
Neoplasms

ASJC Scopus subject areas

  • Medicine(all)
  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

p53 activity is selectively licensed in the Drosophila stem cell compartment. / Wylie, Annika; Lu, Wan Jin; D'Brot, Alejandro; Buszczak, Michael; Abrams, John M.

In: eLife, Vol. 2014, No. 3, e01530, 11.03.2014.

Research output: Contribution to journalArticle

Wylie, Annika ; Lu, Wan Jin ; D'Brot, Alejandro ; Buszczak, Michael ; Abrams, John M. / p53 activity is selectively licensed in the Drosophila stem cell compartment. In: eLife. 2014 ; Vol. 2014, No. 3.
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