p53-dependent upregulation of PIG3 transcription by γ-ray irradiation and its interaction with KAP1 in responding to DNA damage

Xia Qin, Shi Meng Zhang, Bing Li, Xiao Dan Liu, Xing Peng He, Zeng Fu Shang, Qin Zhi Xu, Zeng Qiang Zhao, Qi Nong Ye, Ping Kun Zhou

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

PIG3 (p53-inducible gene 3), originally identified as one of a set of genes induced by p53 before the onset of apoptosis, was assumed to contribute to early cellular response to DNA damage. Here, we studied the relation between p53 status and the increased expression of PIG3 by ionizing radiation (IR), and the related clues regarding the involvement of PIG3 in the cellular response to IR-induced DNA damage signaling. We demonstrated that the pentanucleotide microsatellite sequence was responsible for the p53-dependent induction of PIG3 transcription after irradiation, while sequence upstream of PIG3 promoter could maintain the basal level of expression which was not inducible by irradiation. The interaction of PIG3 and the KRAB-ZFP-associated protein 1 (KAP1), a DNA damage response protein, was revealed. PIG3 nucleus foci were formed 15 min after γ-ray irradiation, and which were found to partially colocalize with the phospho-KAP-1 foci as well as γ-H2AX foci. Although the lac operator tagged EGFP based reporter system revealed that PIG3 does not remodel chromatin in large scale in the cells under normal growing condition, it indeed prompted the chromatin relaxation in the cellular response to DNA damage signaling. All these data suggest that PIG3 is involved in IR-induced DNA damage response, and which maybe partially attribute to its interaction with KAP1.

Original languageEnglish (US)
Pages (from-to)3162-3171
Number of pages10
JournalChinese Science Bulletin
Volume56
Issue number30
DOIs
StatePublished - Oct 2011
Externally publishedYes

Keywords

  • DNA damage response
  • inducible expression
  • KAP1
  • large-scale chromatin remodeling
  • p53
  • PIG3

ASJC Scopus subject areas

  • General

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