p53 is covalently linked to 5.8S rRNA

Beatriz M A Fontoura, Elena A. Sorokina, Ebenezer David, Robert B. Carroll

Research output: Contribution to journalArticle

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Abstract

We report here the isolation and identification of the RNA specifically immunoprecipitated and covalently linked to the tumor suppressor gene product p53. After treatment with proteinase K, the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) band of p53 yields a single, discrete 157-nucleotide RNA, which was cloned, sequenced, and identified as 5.8S rRNA. 5.8S rRNA was obtained only after proteolysis of the p53 SDS-PAGE band. Free 5.8S rRNA did not comigrate with p53 in SDS-PAGE. This RNA was only immunoprecipitated from cells containing p53. Protein-free RNA obtained by proteolysis of the p53 band hybridized to the single-stranded DNA vector containing the antisense sequence of 5.8S rRNA. The covalence of the p53-5.8S rRNA linkage was demonstrated by the following findings: (i) p53 and the linked 5.8S rRNA comigrated in SDS-PAGE; (ii) only after treatment of the p53-RNA complex with proteinase K did the 5.8S rRNA migrate differently from p53-linked 5.8S rRNA; and (iii) this isolated RNA was found linked to phosphoserine, presumably at the 5′ end. Covalent linkage to the single, specific RNA suggests that p53 may be involved in regulating the expression or function of 5.8S rRNA.

Original languageEnglish (US)
Pages (from-to)5145-5151
Number of pages7
JournalMolecular and Cellular Biology
Volume12
Issue number11
StatePublished - Nov 1992

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RNA
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Endopeptidase K
Proteolysis
Phosphoserine
Single-Stranded DNA
Tumor Suppressor Genes
Nucleotides
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Fontoura, B. M. A., Sorokina, E. A., David, E., & Carroll, R. B. (1992). p53 is covalently linked to 5.8S rRNA. Molecular and Cellular Biology, 12(11), 5145-5151.

p53 is covalently linked to 5.8S rRNA. / Fontoura, Beatriz M A; Sorokina, Elena A.; David, Ebenezer; Carroll, Robert B.

In: Molecular and Cellular Biology, Vol. 12, No. 11, 11.1992, p. 5145-5151.

Research output: Contribution to journalArticle

Fontoura, BMA, Sorokina, EA, David, E & Carroll, RB 1992, 'p53 is covalently linked to 5.8S rRNA', Molecular and Cellular Biology, vol. 12, no. 11, pp. 5145-5151.
Fontoura BMA, Sorokina EA, David E, Carroll RB. p53 is covalently linked to 5.8S rRNA. Molecular and Cellular Biology. 1992 Nov;12(11):5145-5151.
Fontoura, Beatriz M A ; Sorokina, Elena A. ; David, Ebenezer ; Carroll, Robert B. / p53 is covalently linked to 5.8S rRNA. In: Molecular and Cellular Biology. 1992 ; Vol. 12, No. 11. pp. 5145-5151.
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