Paper-Based Surface-Enhanced Raman Scattering Lateral Flow Strip for Detection of Neuron-Specific Enolase in Blood Plasma

Xuefei Gao, Peng Zheng, Sujan Kasani, Steven Wu, Feng Yang, Sara Lewis, Sara Nayeem, Elizabeth B. Engler-Chiurazzi, Jane G. Wigginton, James W. Simpkins, Nianqiang Wu

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

An inexpensive and disposable paper-based lateral flow strip (PLFS) has been developed as an immunoassay, in which surface-enhanced Raman scattering (SERS) is utilized for sensing signal transduction. The Au nanostar@Raman Reporter@silica sandwich nanoparticles are developed as the SERS probes, which is the key to the high sensitivity of the device. Compared with a colorimetric PLFS, the SERS-PLFS exhibits superior performance in terms of sensitivity and limit of detection (LOD) in a blood plasma-containing sample matrix. In addition, the SERS-PLFS has been successfully used for detection of neuron-specific enolase (NSE), a traumatic brain injury (TBI) protein biomarker, in diluted blood plasma samples, achieving a LOD of 0.86 ng/mL. Moreover, the SERS-PLFS was successfully employed to measure the NSE level in clinical blood plasma samples taken from deidentified TBI patients. This work demonstrates that the SERS-PLFS has great potential in assisting screening of TBI patients in the point-of-care setting.

Original languageEnglish (US)
Pages (from-to)10104-10110
Number of pages7
JournalAnalytical Chemistry
Volume89
Issue number18
DOIs
StatePublished - Sep 19 2017

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Phosphopyruvate Hydratase
Raman scattering
Blood
Plasmas
Brain
Signal transduction
Biomarkers
Silicon Dioxide
Screening
Nanoparticles
Proteins

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Paper-Based Surface-Enhanced Raman Scattering Lateral Flow Strip for Detection of Neuron-Specific Enolase in Blood Plasma. / Gao, Xuefei; Zheng, Peng; Kasani, Sujan; Wu, Steven; Yang, Feng; Lewis, Sara; Nayeem, Sara; Engler-Chiurazzi, Elizabeth B.; Wigginton, Jane G.; Simpkins, James W.; Wu, Nianqiang.

In: Analytical Chemistry, Vol. 89, No. 18, 19.09.2017, p. 10104-10110.

Research output: Contribution to journalArticle

Gao, X, Zheng, P, Kasani, S, Wu, S, Yang, F, Lewis, S, Nayeem, S, Engler-Chiurazzi, EB, Wigginton, JG, Simpkins, JW & Wu, N 2017, 'Paper-Based Surface-Enhanced Raman Scattering Lateral Flow Strip for Detection of Neuron-Specific Enolase in Blood Plasma', Analytical Chemistry, vol. 89, no. 18, pp. 10104-10110. https://doi.org/10.1021/acs.analchem.7b03015
Gao, Xuefei ; Zheng, Peng ; Kasani, Sujan ; Wu, Steven ; Yang, Feng ; Lewis, Sara ; Nayeem, Sara ; Engler-Chiurazzi, Elizabeth B. ; Wigginton, Jane G. ; Simpkins, James W. ; Wu, Nianqiang. / Paper-Based Surface-Enhanced Raman Scattering Lateral Flow Strip for Detection of Neuron-Specific Enolase in Blood Plasma. In: Analytical Chemistry. 2017 ; Vol. 89, No. 18. pp. 10104-10110.
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