Abstract
An inexpensive and disposable paper-based lateral flow strip (PLFS) has been developed as an immunoassay, in which surface-enhanced Raman scattering (SERS) is utilized for sensing signal transduction. The Au nanostar@Raman Reporter@silica sandwich nanoparticles are developed as the SERS probes, which is the key to the high sensitivity of the device. Compared with a colorimetric PLFS, the SERS-PLFS exhibits superior performance in terms of sensitivity and limit of detection (LOD) in a blood plasma-containing sample matrix. In addition, the SERS-PLFS has been successfully used for detection of neuron-specific enolase (NSE), a traumatic brain injury (TBI) protein biomarker, in diluted blood plasma samples, achieving a LOD of 0.86 ng/mL. Moreover, the SERS-PLFS was successfully employed to measure the NSE level in clinical blood plasma samples taken from deidentified TBI patients. This work demonstrates that the SERS-PLFS has great potential in assisting screening of TBI patients in the point-of-care setting.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 10104-10110 |
| Number of pages | 7 |
| Journal | Analytical Chemistry |
| Volume | 89 |
| Issue number | 18 |
| DOIs | |
| State | Published - Sep 19 2017 |
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ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Paper-Based Surface-Enhanced Raman Scattering Lateral Flow Strip for Detection of Neuron-Specific Enolase in Blood Plasma. / Gao, Xuefei; Zheng, Peng; Kasani, Sujan; Wu, Steven; Yang, Feng; Lewis, Sara; Nayeem, Sara; Engler-Chiurazzi, Elizabeth B.; Wigginton, Jane G.; Simpkins, James W.; Wu, Nianqiang.
In: Analytical Chemistry, Vol. 89, No. 18, 19.09.2017, p. 10104-10110.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Paper-Based Surface-Enhanced Raman Scattering Lateral Flow Strip for Detection of Neuron-Specific Enolase in Blood Plasma
AU - Gao, Xuefei
AU - Zheng, Peng
AU - Kasani, Sujan
AU - Wu, Steven
AU - Yang, Feng
AU - Lewis, Sara
AU - Nayeem, Sara
AU - Engler-Chiurazzi, Elizabeth B.
AU - Wigginton, Jane G.
AU - Simpkins, James W.
AU - Wu, Nianqiang
PY - 2017/9/19
Y1 - 2017/9/19
N2 - An inexpensive and disposable paper-based lateral flow strip (PLFS) has been developed as an immunoassay, in which surface-enhanced Raman scattering (SERS) is utilized for sensing signal transduction. The Au nanostar@Raman Reporter@silica sandwich nanoparticles are developed as the SERS probes, which is the key to the high sensitivity of the device. Compared with a colorimetric PLFS, the SERS-PLFS exhibits superior performance in terms of sensitivity and limit of detection (LOD) in a blood plasma-containing sample matrix. In addition, the SERS-PLFS has been successfully used for detection of neuron-specific enolase (NSE), a traumatic brain injury (TBI) protein biomarker, in diluted blood plasma samples, achieving a LOD of 0.86 ng/mL. Moreover, the SERS-PLFS was successfully employed to measure the NSE level in clinical blood plasma samples taken from deidentified TBI patients. This work demonstrates that the SERS-PLFS has great potential in assisting screening of TBI patients in the point-of-care setting.
AB - An inexpensive and disposable paper-based lateral flow strip (PLFS) has been developed as an immunoassay, in which surface-enhanced Raman scattering (SERS) is utilized for sensing signal transduction. The Au nanostar@Raman Reporter@silica sandwich nanoparticles are developed as the SERS probes, which is the key to the high sensitivity of the device. Compared with a colorimetric PLFS, the SERS-PLFS exhibits superior performance in terms of sensitivity and limit of detection (LOD) in a blood plasma-containing sample matrix. In addition, the SERS-PLFS has been successfully used for detection of neuron-specific enolase (NSE), a traumatic brain injury (TBI) protein biomarker, in diluted blood plasma samples, achieving a LOD of 0.86 ng/mL. Moreover, the SERS-PLFS was successfully employed to measure the NSE level in clinical blood plasma samples taken from deidentified TBI patients. This work demonstrates that the SERS-PLFS has great potential in assisting screening of TBI patients in the point-of-care setting.
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U2 - 10.1021/acs.analchem.7b03015
DO - 10.1021/acs.analchem.7b03015
M3 - Article
VL - 89
SP - 10104
EP - 10110
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 18
ER -