TY - JOUR
T1 - Paper-Based Surface-Enhanced Raman Scattering Lateral Flow Strip for Detection of Neuron-Specific Enolase in Blood Plasma
AU - Gao, Xuefei
AU - Zheng, Peng
AU - Kasani, Sujan
AU - Wu, Steven
AU - Yang, Feng
AU - Lewis, Sara
AU - Nayeem, Sara
AU - Engler-Chiurazzi, Elizabeth B.
AU - Wigginton, Jane G.
AU - Simpkins, James W.
AU - Wu, Nianqiang
N1 - Funding Information:
This work was supported by the National Institute of Neurological Disorders and Stroke of the National Institutes of Health (R15NS087515). The resources provided by the Byars-Tarnay Endowment in the WVU Foundation are also appreciated. The characterization was performed at WVU Shared Facility. In addition, J.G.W. was involved in the RESCUE study in this manuscript that was funded in part by the UT Southwestern High Impact/High Risk Grant, the National Center for Advancing Translational Sciences of the National Institutes of Health under award Number UL1TR001105, and the Resuscitation Outcomes Consortium cooperative agreements (5U01 HL077863-University of Washington Data Coordinating Center, HL077887-University of Texas Southwestern Medical Center in Dallas) from the National Heart, Lung and Blood Institute in partnership with the U.S. Army Medical Research & Material Command and the American Heart Association. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/9/19
Y1 - 2017/9/19
N2 - An inexpensive and disposable paper-based lateral flow strip (PLFS) has been developed as an immunoassay, in which surface-enhanced Raman scattering (SERS) is utilized for sensing signal transduction. The Au nanostar@Raman Reporter@silica sandwich nanoparticles are developed as the SERS probes, which is the key to the high sensitivity of the device. Compared with a colorimetric PLFS, the SERS-PLFS exhibits superior performance in terms of sensitivity and limit of detection (LOD) in a blood plasma-containing sample matrix. In addition, the SERS-PLFS has been successfully used for detection of neuron-specific enolase (NSE), a traumatic brain injury (TBI) protein biomarker, in diluted blood plasma samples, achieving a LOD of 0.86 ng/mL. Moreover, the SERS-PLFS was successfully employed to measure the NSE level in clinical blood plasma samples taken from deidentified TBI patients. This work demonstrates that the SERS-PLFS has great potential in assisting screening of TBI patients in the point-of-care setting.
AB - An inexpensive and disposable paper-based lateral flow strip (PLFS) has been developed as an immunoassay, in which surface-enhanced Raman scattering (SERS) is utilized for sensing signal transduction. The Au nanostar@Raman Reporter@silica sandwich nanoparticles are developed as the SERS probes, which is the key to the high sensitivity of the device. Compared with a colorimetric PLFS, the SERS-PLFS exhibits superior performance in terms of sensitivity and limit of detection (LOD) in a blood plasma-containing sample matrix. In addition, the SERS-PLFS has been successfully used for detection of neuron-specific enolase (NSE), a traumatic brain injury (TBI) protein biomarker, in diluted blood plasma samples, achieving a LOD of 0.86 ng/mL. Moreover, the SERS-PLFS was successfully employed to measure the NSE level in clinical blood plasma samples taken from deidentified TBI patients. This work demonstrates that the SERS-PLFS has great potential in assisting screening of TBI patients in the point-of-care setting.
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U2 - 10.1021/acs.analchem.7b03015
DO - 10.1021/acs.analchem.7b03015
M3 - Article
C2 - 28817769
AN - SCOPUS:85029575211
VL - 89
SP - 10104
EP - 10110
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 18
ER -