Paraffin processing of stented arteries using a postfixation dissolution of metallic and polymeric stents

Ilia Fishbein, Tre Welch, David T. Guerrero, Ivan S. Alferiev, Richard F. Adamo, Michael Chorny, Rohit K. Gupte, Yanqing Tang, Robert J. Levy

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Studying the morphology of the arterial response to endovascular stent implantation requires embedding the explanted stented artery in rigid materials such as poly(methyl methacrylate) to enable sectioning through both the in situ stent and the arterial wall, thus maintaining the proper anatomic relationships. This is a laborious, time-consuming process. Moreover, the technical quality of stained plastic sections is typically suboptimal and, in some cases, precludes immunohistochemical analysis. Here we describe a novel technique for dissolution of metallic and plastic stents that is compatible with subsequent embedding of “destented” arteries in paraffin, fine sectioning, major staining protocols, and immunohistochemistry.

Original languageEnglish (US)
Pages (from-to)483-488
Number of pages6
JournalCardiovascular Pathology
Volume25
Issue number6
DOIs
StatePublished - Nov 1 2016

Keywords

  • Arterial tissue
  • Histological processing
  • Immunohistochemistry
  • Paraffin embedding
  • Stents

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cardiology and Cardiovascular Medicine

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    Fishbein, I., Welch, T., Guerrero, D. T., Alferiev, I. S., Adamo, R. F., Chorny, M., Gupte, R. K., Tang, Y., & Levy, R. J. (2016). Paraffin processing of stented arteries using a postfixation dissolution of metallic and polymeric stents. Cardiovascular Pathology, 25(6), 483-488. https://doi.org/10.1016/j.carpath.2016.08.002