@article{dbb11a526b0544d8a0a77dc29dc06652,
title = "Paraffin processing of stented arteries using a postfixation dissolution of metallic and polymeric stents",
abstract = "Studying the morphology of the arterial response to endovascular stent implantation requires embedding the explanted stented artery in rigid materials such as poly(methyl methacrylate) to enable sectioning through both the in situ stent and the arterial wall, thus maintaining the proper anatomic relationships. This is a laborious, time-consuming process. Moreover, the technical quality of stained plastic sections is typically suboptimal and, in some cases, precludes immunohistochemical analysis. Here we describe a novel technique for dissolution of metallic and plastic stents that is compatible with subsequent embedding of “destented” arteries in paraffin, fine sectioning, major staining protocols, and immunohistochemistry.",
keywords = "Arterial tissue, Histological processing, Immunohistochemistry, Paraffin embedding, Stents",
author = "Ilia Fishbein and Tre Welch and Guerrero, {David T.} and Alferiev, {Ivan S.} and Adamo, {Richard F.} and Michael Chorny and Gupte, {Rohit K.} and Yanqing Tang and Levy, {Robert J.}",
note = "Funding Information: The authors acknowledge Dr. Frederick Schoen, Department of Pathology, Brigham and Women's Hospital, for his review and comments. This work was supported by a grant from the W.W. Smith Charitable Trust to IF (#H-1402), the Children's Hospital of Philadelphia Cardiac Center Research grant (IF), and the NIH NHLBI grant R01-HL111118 (MC). Additional research support was provided by both the William J. Rashkind Endowment and Erin's Fund of the Children's Hospital of Philadelphia. PTA catheters were kindly provided by NuMed (Hopkinton, NY). Authors wish to acknowledge secretarial assistance of Ms. Susan Kerns. Funding Information: This work was supported by a grant from the W.W. Smith Charitable Trust to IF ( #H-1402 ), the Children's Hospital of Philadelphia Cardiac Center Research grant (IF) and by the NIH NHLBI grant R01-HL111118 (MC). Additional research support was provided by both the William J. Rashkind Endowment and Erin's Fund of the Children's Hospital of Philadelphia (RJL). Publisher Copyright: {\textcopyright} 2016 Elsevier Inc.",
year = "2016",
month = nov,
day = "1",
doi = "10.1016/j.carpath.2016.08.002",
language = "English (US)",
volume = "25",
pages = "483--488",
journal = "Cardiovascular Pathology",
issn = "1054-8807",
publisher = "Elsevier Inc.",
number = "6",
}