PARP-1 Regulates Chromatin Structure and Transcription through a KDM5B-Dependent Pathway

Raga Krishnakumar, W. Lee Kraus

Research output: Contribution to journalArticle

168 Citations (Scopus)

Abstract

PARP-1 is an abundant nuclear enzyme that regulates gene expression, although the underlying mechanisms are unclear. We examined the interplay between PARP-1, histone 3 lysine 4 trimethylation (H3K4me3), and linker histone H1 in the chromatin-dependent control of transcription. We show that PARP-1 is required for a series of molecular outcomes at the promoters of PARP-1-regulated genes, leading to a permissive chromatin environment that allows loading of the RNA Pol II machinery. PARP-1 does so by (1) preventing demethylation of H3K4me3 through the PARylation, inhibition, and exclusion of the histone demethylase KDM5B; and (2) promoting the exclusion of H1 and the opening of promoter chromatin. Upon depletion of PARP-1, these outcomes do not occur efficiently. Interestingly, cellular signaling pathways can use the regulated depletion of PARP-1 to modulate these chromatin-related molecular outcomes. Collectively, our results help to elucidate the roles of PARP-1 in the regulation of chromatin structure and transcription.

Original languageEnglish (US)
Pages (from-to)736-749
Number of pages14
JournalMolecular Cell
Volume39
Issue number5
DOIs
StatePublished - Sep 2010

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Chromatin
Histones
Histone Demethylases
RNA Polymerase II
Lysine
Gene Expression
Enzymes
Genes

Keywords

  • DNA
  • Proteins
  • Signaling

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Medicine(all)

Cite this

PARP-1 Regulates Chromatin Structure and Transcription through a KDM5B-Dependent Pathway. / Krishnakumar, Raga; Kraus, W. Lee.

In: Molecular Cell, Vol. 39, No. 5, 09.2010, p. 736-749.

Research output: Contribution to journalArticle

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