Partial biochemical maturation of aneurally cultured human skeletal muscle

S. T. Iannaccone, B. Nagy, F. J. Samaha

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

We studeid cultures of human skeltal muscle in vitro and established standards for biochemical markers of cellular differentiation. DNA synthesis ceased at the time of fusion, implying the absence of fibroblasts. Myosin heavy-chain synthesis, creatine and pyruvate kinase activities, and isoenzymes of creatine kinase were measured serially over 36 days. Filamin and fibronectin proteins were identified in these cultures. Compared to chick muscle in culture, human skeletal muscle cells remained relatively immature. These data provide a basis for the study of diseased human muscle cells in culture.

Original languageEnglish (US)
Pages (from-to)846-851
Number of pages6
JournalNeurology
Volume32
Issue number8
DOIs
StatePublished - Aug 1982

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ASJC Scopus subject areas

  • Clinical Neurology

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