Partial correction of murine hereditary growth disorder by germ-line incorporation of a new gene

Robert E Hammer, Richard D. Palmiter, Ralph L. Brinster

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

The dwarf little (lit) mouse is a model for the human hereditary disorder, isolated growth hormone (GH) deficiency type I1. In these animals, dwarfism results from an autosomal recessively inherited gene mutation. The GH gene is present2 but production of GH mRNA is deficient3, resulting in reduced serum GH and concomitantly decreased serum somatomedin 4-6. Growth retardation is evident by 15 days of age and adult animals reach approximately one-half normal size1. Mutant mice of both sexes also exhibit a delayed onset of puberty, with males having a high degree of infertility1. As administration of GH restores growth 7, we reasoned that growth failure in the mutant mice might be corrected by providing them with sufficient GH by gene therapy. Here we demonstrate that although the rat and human GH genes alone do not restore growth in transgenic mutants, a metallothionein-rat growth hormone fusion gene (MT-rGH) does. Moreover, the fertility of transgenic mutant males is improved; however, female fertility is impaired.

Original languageEnglish (US)
Pages (from-to)65-67
Number of pages3
JournalNature
Volume311
Issue number5981
DOIs
StatePublished - 1984

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Growth Disorders
Germ Cells
Growth Hormone
Genes
Growth
Fertility
Pituitary Dwarfism
Delayed Puberty
Dwarfism
Human Growth Hormone
Metallothionein
Gene Fusion
Somatomedins
Serum
Genetic Therapy
Messenger RNA
Mutation

ASJC Scopus subject areas

  • General

Cite this

Partial correction of murine hereditary growth disorder by germ-line incorporation of a new gene. / Hammer, Robert E; Palmiter, Richard D.; Brinster, Ralph L.

In: Nature, Vol. 311, No. 5981, 1984, p. 65-67.

Research output: Contribution to journalArticle

Hammer, Robert E ; Palmiter, Richard D. ; Brinster, Ralph L. / Partial correction of murine hereditary growth disorder by germ-line incorporation of a new gene. In: Nature. 1984 ; Vol. 311, No. 5981. pp. 65-67.
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