Partial isolation and characterization of antigen(s) associated with murine melanoma

J. C. Bystryn, I. Schenkein, S. Baur, J. W. Uhr

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Antigens associated with murine B16 melanoma (MAA) were radiolabeled by the incubation of tumor cells with 3H leucine and identified by an antigen binding radioimmunoassay. The MAA had a molecular weight of 150,000 to 200,000 daltons, were precipitable by 52% ammonium sulfate, and were inactivated by treatment with heat, acid, base, trypsin, or papain. Treatment with neuraminidase greatly increased MAA antigenicity. These results suggest that MAA are glycoproteins. Some MAA were present to a lesser and variable extent in normal syngeneic murine fibroblasts. MAA were released into culture medium by tumor cells and to a lesser extent by normal cells.

Original languageEnglish (US)
Pages (from-to)1263-1269
Number of pages7
JournalJournal of the National Cancer Institute
Volume52
Issue number4
StatePublished - 1974

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Melanoma
Antigens
Experimental Melanomas
Papain
Ammonium Sulfate
Neuraminidase
Leucine
Trypsin
Radioimmunoassay
Culture Media
Neoplasms
Glycoproteins
Fibroblasts
Hot Temperature
Molecular Weight
Acids

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Bystryn, J. C., Schenkein, I., Baur, S., & Uhr, J. W. (1974). Partial isolation and characterization of antigen(s) associated with murine melanoma. Journal of the National Cancer Institute, 52(4), 1263-1269.

Partial isolation and characterization of antigen(s) associated with murine melanoma. / Bystryn, J. C.; Schenkein, I.; Baur, S.; Uhr, J. W.

In: Journal of the National Cancer Institute, Vol. 52, No. 4, 1974, p. 1263-1269.

Research output: Contribution to journalArticle

Bystryn, JC, Schenkein, I, Baur, S & Uhr, JW 1974, 'Partial isolation and characterization of antigen(s) associated with murine melanoma', Journal of the National Cancer Institute, vol. 52, no. 4, pp. 1263-1269.
Bystryn, J. C. ; Schenkein, I. ; Baur, S. ; Uhr, J. W. / Partial isolation and characterization of antigen(s) associated with murine melanoma. In: Journal of the National Cancer Institute. 1974 ; Vol. 52, No. 4. pp. 1263-1269.
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