TY - JOUR
T1 - Partitioning of the matrix fraction of the golgi apparatus during mitosis in animal cells
AU - Seemann, Joachim
AU - Pypaert, Marc
AU - Taguchi, Tomohiko
AU - Malsam, Jörg
AU - Warren, Graham
PY - 2002/2/1
Y1 - 2002/2/1
N2 - The Golgi apparatus is partitioned during mitosis in animal cells by a process of fragmentation, dispersal, and reassembly in each daughter cell. We fractionated the Golgi apparatus in vivo using the drug brefeldin A or a dominant-negative mutant of the Sar1p protein. After these treatments, Golgi enzymes moved back to the endoplasmic reticulum, leaving behind a matrix of Golgi structural proteins. Under these conditions, cells still entered and exited mitosis normally, and their Golgi matrix partitioned in a manner very similar to that of the complete organelle. Thus, the matrix may be the partitioning unit of the Golgi apparatus and may carry the Golgi enzyme-containing membranes into the daughter cells.
AB - The Golgi apparatus is partitioned during mitosis in animal cells by a process of fragmentation, dispersal, and reassembly in each daughter cell. We fractionated the Golgi apparatus in vivo using the drug brefeldin A or a dominant-negative mutant of the Sar1p protein. After these treatments, Golgi enzymes moved back to the endoplasmic reticulum, leaving behind a matrix of Golgi structural proteins. Under these conditions, cells still entered and exited mitosis normally, and their Golgi matrix partitioned in a manner very similar to that of the complete organelle. Thus, the matrix may be the partitioning unit of the Golgi apparatus and may carry the Golgi enzyme-containing membranes into the daughter cells.
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U2 - 10.1126/science.1068064
DO - 10.1126/science.1068064
M3 - Article
C2 - 11823640
AN - SCOPUS:0036468386
SN - 0036-8075
VL - 295
SP - 848
EP - 851
JO - Science
JF - Science
IS - 5556
ER -