TY - JOUR
T1 - Pathogen reduction in human plasma using an ultrashort pulsed laser
AU - Tsen, Shaw Wei D.
AU - Kingsley, David H.
AU - Kibler, Karen
AU - Jacobs, Bert
AU - Sizemore, Sara
AU - Vaiana, Sara M.
AU - Anderson, Jeanne
AU - Tsen, Kong Thon
AU - Achilefu, Samuel
PY - 2014/11/5
Y1 - 2014/11/5
N2 - Pathogen reduction is a viable approach to ensure the continued safety of the blood supply against emerging pathogens. However, the currently licensed pathogen reduction techniques are ineffective against non-enveloped viruses such as hepatitis A virus, and they introduce chemicals with concerns of side effects which prevent their widespread use. In this report, we demonstrate the inactivation of both enveloped and non-enveloped viruses in human plasma using a novel chemical-free method, a visible ultrashort pulsed laser. We found that laser treatment resulted in 2-log, 1-log, and 3-log reductions in human immunodeficiency virus, hepatitis A virus, and murine cytomegalovirus in human plasma, respectively. Laser-treated plasma showed ≥70% retention for most coagulation factors tested. Furthermore, laser treatment did not alter the structure of a model coagulation factor, fibrinogen. Ultrashort pulsed lasers are a promising new method for chemical-free, broad-spectrum pathogen reduction in human plasma.
AB - Pathogen reduction is a viable approach to ensure the continued safety of the blood supply against emerging pathogens. However, the currently licensed pathogen reduction techniques are ineffective against non-enveloped viruses such as hepatitis A virus, and they introduce chemicals with concerns of side effects which prevent their widespread use. In this report, we demonstrate the inactivation of both enveloped and non-enveloped viruses in human plasma using a novel chemical-free method, a visible ultrashort pulsed laser. We found that laser treatment resulted in 2-log, 1-log, and 3-log reductions in human immunodeficiency virus, hepatitis A virus, and murine cytomegalovirus in human plasma, respectively. Laser-treated plasma showed ≥70% retention for most coagulation factors tested. Furthermore, laser treatment did not alter the structure of a model coagulation factor, fibrinogen. Ultrashort pulsed lasers are a promising new method for chemical-free, broad-spectrum pathogen reduction in human plasma.
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U2 - 10.1371/journal.pone.0111673
DO - 10.1371/journal.pone.0111673
M3 - Article
C2 - 25372037
AN - SCOPUS:84910630789
SN - 1932-6203
VL - 9
JO - PloS one
JF - PloS one
IS - 11
M1 - e111673
ER -