TY - JOUR
T1 - Pathways of cholesterol crystallization in model bile and native bile
AU - Portincasa, P.
AU - Moschetta, A.
AU - Van Erpecum, K. J.
AU - Calamita, G.
AU - Margari, A.
AU - VanBerge-Henegouwen, G. P.
AU - Palasciano, G.
PY - 2003/2
Y1 - 2003/2
N2 - Hypersecretion of hepatic cholesterol, chronic supersaturation of bile with cholesterol and rapid precipitation of cholesterol crystals in the gallbladder from cholesterol-enriched vesicles represent the primum movens in cholesterol gallstone formation. Physical-chemical factors and pathways leading to cholesterol crystallization can be investigated in artificial model biles and ex vivo in fresh human bile. Depending on modulatory factors (i.e., lipid concentration, bile salt or phospholipid species, humidity, mucins, etc.), cholesterol can precipitate in several forms (i.e., monohydrate, anhydrous) and habits (i.e., plate-like, needle-like, intermediate arcs, filaments, tubules, spirals). Careful analysis of biliary cholesterol crystals includes biochemical analysis of precipitated crystals, polarizing quantitative light microscopy, and turbidimetric methods. In this paper, recent concepts on cholesterol crystallization in artificial model biles as well as in human bile will be reviewed.
AB - Hypersecretion of hepatic cholesterol, chronic supersaturation of bile with cholesterol and rapid precipitation of cholesterol crystals in the gallbladder from cholesterol-enriched vesicles represent the primum movens in cholesterol gallstone formation. Physical-chemical factors and pathways leading to cholesterol crystallization can be investigated in artificial model biles and ex vivo in fresh human bile. Depending on modulatory factors (i.e., lipid concentration, bile salt or phospholipid species, humidity, mucins, etc.), cholesterol can precipitate in several forms (i.e., monohydrate, anhydrous) and habits (i.e., plate-like, needle-like, intermediate arcs, filaments, tubules, spirals). Careful analysis of biliary cholesterol crystals includes biochemical analysis of precipitated crystals, polarizing quantitative light microscopy, and turbidimetric methods. In this paper, recent concepts on cholesterol crystallization in artificial model biles as well as in human bile will be reviewed.
KW - Bile lipids
KW - Cholesterol carriers
KW - Gallstones
KW - Nephelometry
KW - Turbidimetric methods
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U2 - 10.1016/S1590-8658(03)00009-4
DO - 10.1016/S1590-8658(03)00009-4
M3 - Article
C2 - 12747631
AN - SCOPUS:0042768056
SN - 1590-8658
VL - 35
SP - 118
EP - 126
JO - Digestive and Liver Disease
JF - Digestive and Liver Disease
IS - 2
ER -