Patt1, a novel protein acetyltransferase that is highly expressed in liver and downregulated in hepatocellular carcinoma, enhances apoptosis of hepatoma cells

Zhen Liu, Yang Liu, Huiqiang Wang, Xinjian Ge, Qihuang Jin, Guanghui Ding, Yanan Hu, Ben Zhou, Zhongjian Chen, Xuemei Ge, Baohua Zhang, Xiaobo Man, Qiwei Zhai

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Protein acetylation is increasingly recognized as an important post-translational modification. Although a lot of protein acetyltransferases have been identified, a few putative acetyltransferases are yet to be studied. In this study, we identified a novel protein acetyltransferase, Patt1, which belongs to GNAT family. Patt1 exhibited histone acetyltransferase activity and auto-acetylation activity. Deletion and mutation analysis of the predicted acetyltransferase domain in Patt1 showed that the conserved Glu139 was an important residue for its protein acetyltransferase activity. Furthermore, we found that Patt1 was highly expressed in liver and significantly downregulated in hepatocellular carcinoma tissues. In addition, we showed that overexpression of Patt1 enhanced the apoptosis of hepatoma cells dependent on its acetyltransferase activity, whereas knockdown of Patt1 significantly protected Chang liver cells from apoptosis. These data suggest that Patt1 might be involved in the development of hepatocellular carcinoma, and could be served as a potential therapy target for hepatocellular carcinoma.

Original languageEnglish (US)
Pages (from-to)2528-2537
Number of pages10
JournalInternational Journal of Biochemistry and Cell Biology
Volume41
Issue number12
DOIs
StatePublished - Dec 1 2009

Keywords

  • Acetyltransferase
  • Apoptosis
  • GNAT family
  • Hepatocellular carcinoma
  • Liver

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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